Capillary supercritical fluid chromatography/mass spectrometric

Mobile fluid interaction with the stationary phase in SFC was investigated with mass spectrometric tracer pulse chromatography (96). Using capillary supercritical fluid chromatography, the effect of methanol as an additive was studied on the partition behavior of n-pentane into 5 % phenylmethylsilicone stationary phase. The results showed that the mobile fluid uptake by the stationary phase decreased with increasing temperature and pressure. Thus suggests that stationary phase swelling, may occur in SFC. 

Pinkston, J.D. et al., Evaluation of capillary supercritical fluid chromatography with mass spectrometric detection for the analysis of a drug (mebeverine) in a dog plasma matrix, J. Chromatogr., 622(2), 209, 1993. 

Since NIV occurs as a co-contaminant with other trichothecene mycotoxins, it is often analyzed simultaneously with the co-contaminants rather than alone. Analytical methods developed so far include thin layer chromatography (TLC) capillary gas chromatography (GC) with electron-capture detection (BCD), flame ionization detection (FID), or mass spectrometric detection (GC/MS) high-performance liquid chromatography (HPLC) with ultraviolet (UV), fluorescence, or mass spectrometric detection supercritical fluid chromatography (SFC) and time-of-flight mass spectrometry (LC/TOF-MS). 

The on-line combination of a chromatographic separation technique (GC, LC, but also thin-layer chromatography (TLC), capillary zone electrophoresis (CZE) and supercritical fluid chromatography (SFC)) with MS enables the mass spectrometric characterization of components in complex mixtures after separation with minimal or no sample loss. It is especially useful in the identification of minor or trace components that are difficult to collect by fractionation of the column effluent or would be easily lost. Furthermore, as already indicated above, on-line GC-MS and LC-MS-MS are important tools in quantitative bioanalysis as well. Obviously, fractionation in large series of samples for routine quantitative applications would be extremely time-consuming and ineffective. 

Several types of liquid chromatography (LC) and one nonchromatografiiic separation system for liquids have been interfaced with MS. High-performance LC (HPLC) is widely used to separate nonvolatile organic compounds of aU polarities and MWs. Coupled to a mass spectrometer, the technique is called LC-MS. Supercritical fluid chromatogr hy (SFC) and the nonchromatographic separation technique of capillary electrophoresis (CE) are also used with mass spectrometric detection. The interfacing, ionization sources, operation, and plications of these hyphenated methods are covered in Chapters 10 and 13. 

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