Capillary electrophoresis peptides

Non-denaturing gel electrophoresis Denaturing (SDS) gel electrophoresis Two-dimensional electrophoresis Capillary electrophoresis Peptide mapping HPLC (mainly RP-HPLC)... 

A. J. Tomlinson and S. Naylor, Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry foi analysis of peptide mixtures, J. Capillary Electrophoresis 225-233 (1995). 

P. D. Grossman, J. C. Colburn, H. H. Lauer, R. G. Nielsen, R. M. Riggin, G. S. Sittampalam and E. C. Rickard, Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptides . Anal. Chem. 61 1186-1194 (1989). 

M. Stromqvist, Peptide mapping using combinations of size-exclusion chromatography, reversed-phase chromatography and capillary electrophoresis , 7. Chromatogr. 667 304-310(1994). 

J. P. Larmann-Jr, A. V. Lemmo, A. W. Moore and J. W. Jorgenson, Two-dimensional sep-ar ations of peptides and proteins by comprehensive liquid chromatography-capillary electrophoresis . Electrophoresis 14 439-447 (1993). 

Figure 11.18 Schematic diagram of an in-line SPE unit for CE using (a) polyester wool frits to hold the sorbent, or (b) a paiticle-loaded membrane. Reprinted from Journal of Capillary Electrophoresis, 2, A. J. Tomlinson and S. Naylor, Enhanced performance membrane preconcenti ation-capillary electrophoresis-mass spectiometi y (mPC-CE-MS) in conjunction with ti ansient isotachophoresis for analysis of peptide mixtures, pp 225-233, 1995, with permission from ISC Teclmical Publications Inc.

High performance capillary electrophoresis was introduced originally as an analytical tool. Now that instruments are equipped with automated fraction collection, however, capillary electrophoresis can be used for micropreparative collection of individual peaks separated from a mixture. Using the fraction collection feature, nanomolar amounts of solute such as proteins, peptides, oligonucleotides can be collected in amounts sufficient for microsequencing. An intersample washing procedure and use of well-formed capillaries aid in the prevention of artifacts.44... 

Boss, H. J., Rohde, M. F., and Rush, R. S., Multiple sequential fraction collection of peptides and glycopeptides by high-performance capillary electrophoresis, Anal. Biochem., 230, 123, 1995. 

Advis, J. P., Hernandez, L., and Guzman, N. A., Analysis of brain neuropeptides by capillary electrophoresis determination of luteinizing hormone-re-leasing hormone from ovine hypothalamus, Peptide Res., 2, 389, 1989. 

Chu, Y.-H. and Whitesides, G. M., Affinity capillary electrophoresis can simultaneously measure binding constants of multiple peptides to vancomycin,. Org. Chem., 57, 3524, 1992. 

Satow, T., Machida, A., Funakushi, K., and Palmieri, R., Effects of the sample matrix on the separation of peptides by high performance capillary electrophoresis, HRC CC, 14, 276, 1991. 

Grossman, P. D., Colburn, J. C., and Lauer, H. H., A semiempirical model for the electrophoretic mobilities of peptides in tree-solution capillary electrophoresis, Anal. Biochem., 179, 28, 1989. 

Palmieri, R., Peptide mapping by capillary electrophoresis, Appl. Brief DS-774, Beckman Instruments, Fullerton, CA, 1990. 

Rickard, E. C., Strohl, M. M., and Nielsen, R. G., Correlation of electrophoretic mobilities from capillary electrophoresis with physicochemical properties of proteins and peptides, Anal. Biochem., 197, 197, 1991. 

Simple etching of the capillary end served to decouple the electrophoretic current from that of amperometric detection, permitting quantitation of attomole levels of catecholamines from brain microdialyzates.24 A postcolumn reactor using bromine generated electrochemically in situ has been used in the detection of peptide thiols, such as glutathione and cysteine, separated by capillary electrophoresis.25... 

Bonneil, E., Mercier, M. and Waldron, K.C., Reproducibility of a solid-phase trypsin microreactor for peptide mapping by capillary electrophoresis, Anal. Chim. Acta, 404, 29, 2000. 

Li, J., Kelly, J.F., Chernushevich, I., Harrison, D.J., and Thibault P., Separation and identification of peptides from gel-isolated membrane proteins using a microfabricated device for combined capillary electrophoresis/nanoelectro-spray mass spectrometry, Anal. Chem. 72, 599, 2000. 

Baars, M.J. and Patonay, G., Ultrasensitive detection of closely related angiotensin I peptides using capillary electrophoresis with near-infrared laser-induced fluorescence detection, Anal. Chem. 71, 667, 1999. 

Capillary Liquid Chromatography Coupled with Capillary Electrophoresis for Analysis of Unlabeled Peptides and Proteins... 

Karim, M.R., Shinagawa, S., Takagi, T. (1994). Electrophoretic mobilities of the complexes between sodium dodecyl sulfate and various peptides or proteins determined by free solution electrophoresis using coated capillaries. Electrophoresis 15, 1141-1146. 

High performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are two instrumental separation techniques that are applicable to the separation of proteins and peptides. The advantage of HPLC and CE techniques is that they afford the analyst the freedom to resolve a complex mixture by different routes employing different... 

Amini, A., Chakraborty, A., Regnier, F.E. (2002). Simplification of complex tryptic digests for capillary electrophoresis by affinity selection of histidine-containing peptides with immobilized metal ion affinity chromatography. J. Chromatogr. B 772, 35-44. 

Janini, G.M., Zhou, M., Yu, L.-R., Blonder, J., Gignac, M., Comads, T.P., Issaq, H.J., Veenstra, T.D. (2003). On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry evaluation for peptide analysis and protein identification. Anal. Chem. 75, 5984—5993. 

Larmann, J.P., Lemmo, A.V., Moore, A.W., Jorgenson, J.W. (1993). Two-dimensional separations of peptides and proteins hy comprehensive liquid chromatography-capillary electrophoresis. Electrophoresis 14, 439 147. 

Sanz-Nebot, V., Benavente, F., Barbosa, J. (2002). Liquid chromatography-mass spectrometry and capillary electrophoresis combined approach for separation and characterization of multicomponent peptide mixtures application to crude products of leuprolide synthesis. J. Chromatogr. A 950, 99-111. 

Due to the fact that biarsenical-TC complex is stable under the denaturing conditions typically used for gel electrophoresis of proteins and has a molecular weight of less than 2 KDa, when bonded to the biarsenical dye [157], Kottegoda and collaborators [161] studied the biarsenical dyes, as fluorescent probes for in vitro, cellular peptide, and proteins studies using capillary electrophoresis. 

Zhang H, Le Potier I, Smadja C et al (2006) Fluorescent detection of peptides and amino acids for capillary electrophoresis via on-line derivatization with 4-fluoro-7-nitro-2,l, 3-benzoxadiazole. Anal Bioanal Chem 386 1387-1394... 

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