Capillary electrophoresis matrix assisted laser

Preisler, J., Hu, R, Rejtar, T. and Karger, B.L. (2000) Capillary-electrophoresis-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a vacuum deposition interface. Anal. Chem. 72,4785 795. 

Figure 2.1 Mass spectrometric approach. Dl, direct inlet GC, gas chromatography HPLC, high performance liquid chromatography CZE, capillary zone electrophoresis El, electron ionization Cl, chemical ionization ESI, electrospray ionization DESI, desorption electrospray ionization APCI, atmospheric pressure chemical ionization MALDI, matrix assisted laser desorption ionization B, magnetic analyzer E, electrostatic analyzer...

DGE a AC AMS APCI API AP-MALDI APPI ASAP BIRD c CAD CE CF CF-FAB Cl CID cw CZE Da DAPCI DART DC DE DESI DIOS DTIMS EC ECD El ELDI EM ESI ETD eV f FAB FAIMS FD FI FT FTICR two-dimensional gel electrophoresis atto, 10 18 alternating current accelerator mass spectrometry atmospheric pressure chemical ionization atmospheric pressure ionization atmospheric pressure matrix-assisted laser desorption/ionization atmospheric pressure photoionization atmospheric-pressure solids analysis probe blackbody infrared radiative dissociation centi, 10-2 collision-activated dissociation capillary electrophoresis continuous flow continuous flow fast atom bombardment chemical ionization collision-induced dissociation continuous wave capillary zone electrophoresis dalton desorption atmospheric pressure chemical ionization direct analysis in real time direct current delayed extraction desorption electrospray ionization desorption/ionization on silicon drift tube ion mobility spectrometry electrochromatography electron capture dissociation electron ionization electrospray-assisted laser desorption/ionization electron multiplier electrospray ionization electron transfer dissociation electron volt femto, 1CT15 fast atom bombardment field asymmetric waveform ion mobility spectrometry field desorption field ionization Fourier transform Fourier transform ion cyclotron resonance... 

Stutz, H. (2005). Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix-assisted laser desorption/ionization and electrospray—mass spectrometry detection. Electrophoresis 26, 1254—1290. 

Zhou, G. H., Luo, G. A., Zhou, Y, Zhou, K. Y, Zhang, X. D., and Huang, L. Q. (1998). Application of capillary electrophoresis, liquid chromatography, electrospray mass spectrometry and matrix-assisted laser desorption/ionization time of flight mass spectrometry to the characterization of recombinant human erythropoietin. Electrophoresis 19, 2348—2355. 

Huck, C. W., Bakry, R., Huber, L. A., and Bonn, G. K. (2006). Progress in capillary electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Electrophoresis 27, 2063—2074. 

Yeung, K. K. C., Kiceniuk, A. G., and Li, L. (2001). Capillary electrophoresis using a surfactant-treated capillary coupled with offline matrix-assisted laser desorption ionization mass spectrometry for high efficiency and sensitivity detection of proteins. /. Chromatogr. A 931, 153 — 162. 

Chakel, J. A., Erno, J., Hancock, W. S., and Swedberg, S. A. (1997). Analysis of recombinant DNA-derived glycoproteins via high-performance capillary electrophoresis coupled with off-line matrix-assisted laser desorption ionization time-of-fiight mass spectrometry. /. Chromatogr. B 689, 215—220. 

Page, J. S., Rubakhin, S. S., and Sweedler, J. V. (2000). Direct cellular assays using off-line capillary electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analyst 125, 555-562. 

Suzuki, H., Muller, O., Guttman, A., and Karger, B. L. (1997). Analysis of 1-aminopyrene 3,6,8-trisulfonate-derivatized oligosaccharides by capillary electrophoresis with matrix- assisted laser desorptlon/ionization tlme-of-fhght mass spectrometry. Anal. Chem. 69, 4554—4559. 

Zhang, H., and Caprioli, R. M. (1996). Capillary electrophoresis combined with matrix-assisted laser desorption/ionizatlon mass spectrometry continuous sample deposition on a matrix-precoated membrane target. /. Mass Spectrom. 31, 1039—1046. 

Rubakhin, S.S., Page, J.S., Monroe, B.R., Sweedler, J.V. (2001). Analysis of cellular release using capillary electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis, 22(17), 3752-3758. 

Apffel, A. Chakel, J. Udiavar, S. Hancock, W. S. Souders, C. Pungor, E. Jr. 1995. Application of capillary electrophoresis, high-performance liquid chromatography, on-line electrospray mass spectrometry and matrix-assisted laser desorption ionization—time of flight mass spectrometry to the characterization of single-chain plasminogen activator. . /. Chromatogr. A, 717,41-60. 

Elaboration of nerve agent toxicokinetics requires sophisticated analytical tools to detect and, if possible, to quantify the free toxicants as well as adducts with proteins and enzymes. Analysis of OP nerve agents has been performed by capillary electrophoresis (CE), biosensors, matrix-assisted laser desorption/ionization (MALDI) MS, desorption electrospray ionization MS (DESI MS), ion mobility time-of-flight MS (IM-TOF MS), nuclear magnetic resonance spectroscopy (NMR), LC-UV, gas chromatography (GC), and many more techniques (Hooijschuur et al, 2002 John et al, 2008). 

Blakley, C.R., Vestal, M.L., Verentchikov, A. and Wang, Y.P. (1994) Liquid Chromatograph and Capillary Electrophoresis Interfaces for Matrix Assisted Laser Desorption Time-of-flight Mass Spectrometry. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, p. 1045. 

Castoro, J.A., Chiu, R.W., Monnig, C.A. and Wilkins, C.L. (1992) Matrix-assisted laser desorp-tion/ionization of capillary electrophoresis effluents by fourier transform mass spectrometry. J. Am. Chem. Soc. 114, 7571-7572. 

Van Veelen, P.A., Tjaden, U.R., VanderGieef, J., Ingendoh, A.and Hillenkamp, F. (1993) Off-line coupling of capillary electrophoresis with matrix-assisted laser desorption mass spectrometry. J. Chromatogr. 647,367-374. 

Capillary electrophoresis has been used to separate and analyze synthetically modified carbohydrates in the nanogram to milligram range [163]. Ruorophore-assisted capillary electrophoresis (FACE) has successfully been used to separate nanogram amounts of maltodextrins, containing 4—76 D-glucose residues [164,165,166], see Fig. 9. Many carbohydrates can be analytically separated by matrix-assisted, laser desorption, ionization-time of flight. 

Mass spectrometry is another detection technique widely used in neuropeptide analysis. Concentration sensitivities in CE-MS do not reach those obtained by CE-LIF nevertheless, tedious derivatization procedures are avoided. In addition, CE-MS has proven to be a powerful tool for structure elucidation as illustrated by the investigation of the in vivo metabolic fate of peptide E by Caprioli s group [12]. After microdialysis and in-line SPE, neuropeptides migrating out of the electrophoresis capillary were deposited directly onto a precoated cellulose target used in matrix-assisted laser desorption-time of flight (MALDI-TOF) MS subsequently. Structural information is then obtained along with the mass of the peptide(s). 

Table 8.3. Representative bulk methods of organic characterization MALDl, matrix-assisted laser desorption ionization MS, mass spectrometry CE, capillary electrophoresis. ... 

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