Calcium in serum

Pybus, J. Feldman, F. J. and Bowers, 6. N., Jr. Measurement of total calcium in serum by atomic absorption spectrophotmetry with use of a strontiim internal reference. Clin. Chem. (1970), lj6, 998 - 1007. [Pg.151]

Cali JP, Mandel J, Moore L, and Young DS (1972) A Referee Method for the Determination of Calcium in Serum Standard Reference Materials. NBS Spec Publ 260-36. National Bureau of Standards, Washington, DC. [Pg.102]

The most important applications of Ca " ISEs are in biomedical practice, especially in the determination of ionized calcium in serum. A pioneer work in this field was that by Moore [149] (see also [110,111]). At present the Ca ISE is used in the solvent polymeric version in a number of automatic devices for determining ionized calcium in serum, usually with periodic recalibration of the electrode and thermostatting to 37 °C. It should be noted that ISEs measure... [Pg.189]

Reference Method for the Determination of Calcium in Serum,"Natlonal Bureau of Standards Special Publication 260-36, 1972. [Pg.23]

K4. Kingsley, G. R., and Schaffert, R. R., Micro-flame photometric determination of sodium, potassium, and calcium in serum with organic solvents. J. Biol. Chem. 206, 807-815 (1954). [Pg.58]

Z3. Zettner, A., and Seligson, D., Application of atomic absorption spectrophotometry in the determination of calcium in serum. Clin. Chem. 10, 869-890 (19M). [Pg.62]

About 40% of senun calciiun is protein boxmd, with most of it (80%) being bound to albumin. Generally, one or two calcium ions are associated with serum albumin. Albumin serves as a calcium buffer. It can bind more calcium ions when excessive concentrations of calcium appear in the bloodstream. About 13% of the calcium in serum is weakly complexed with phosphate, citrate, and sulfate. About half (47%) of serum calcium occurs as the free calcium ion. The level of free serum Ca is maintained within narrow limits, 1.0 to 1.25 mM (40 to 50 pg/ml). The normal concentration of total serum calcium (boimd plus free) is 85 to 105 pg/ml. Conditions in which the level of free serum calcium falls below and rises above the normal range are called hypocalcemia and hypercalcemia, respectively. The term ionized calcium is often used to refer to the concentrations of free calcium. This term is not scientifically accurate, because all of the calcium in the body is ionized. Calcium does not engage in the formation of covalent bonds (Cotton and Wilkinson, 1966). [Pg.782]

E520 Gawoski, J.M. and Walsh, D. (1989). Citrate interference in assays of total calcium in serum. Clin. Chem. 35, 2140-2141. [Pg.299]

National Committee for Clinical Laboratory Standards. A designated comparison method for the measurement of ionized calcium in serum Approved Standard. NCCLS Document C39-A, Wayne PA NCCLS, 2000. [Pg.118]

KaUner A. Preanalytical procedures in the measurement of ionized calcium in serum and plasma. Eur J Clin Chem Clin Biochem 1996 34 53-8. [Pg.473]

Figure 49-2 Equilibria and determinations of calcium in serum. Calcium can move among three physiochemical pools (1) free calcium, (2) protein-bound calcium, and (3) calcium complexed with inorganic and organic anions. Methods for determining total calcium measure all three pools, whereas methods for determining free calcium measure only that pool.

Photometric and atomic absorption spectrophotometric methods for measuring the total calcium in serum and plasma are described and discussed below. [Pg.1897]

Reference intervals for Total and Free Calcium IN Serum and Plasma Total Calcium... [Pg.1903]

Boink ABT, Buckley BM, Cristiansen TF, Covington AK, Maas A, Mueller-Plathe O, et al. Reference method for determination of ionized calcium in serum, plasma or whole blood. In Maas AHJ, ed. Methodology and clinical applications of ion selective electrodes. Copenhagen International Federation of Clinical Chemistry, 1986 39-61. [Pg.1945]

Bowers GN Jr, Brassad C, Sena SR Measurement of ionized calcium in serum with ion-selective electrodes a mature technology that can meet the daily service needs. Clin Chem 1986 32 1437-44. [Pg.1946]

Cali JP, Mandel J, Moore L, Young DS. A referee method for the determination of calcium in serum. NBS Special Publication 1972 260-236 ... [Pg.1947]

D Orazio P, Bowers GN Jr. Design and preliminary performance characteristics of a newly proposed reference cell for ionized calcium in serum. Clin Chem 1992 38 1332-9. [Pg.1948]

Larsson L, Ohman S. Effect of sihcone-separator tubes and storage time on ionized calcium in serum. Clin Chem 1985 31 169-70. [Pg.1955]

Leary NO, Pembroke A, Duggan PF. Single stable reagent (Arsenazo III) for optically robust measurement of calcium in serum and plasma. Clin Chem 1992 38 904-8. [Pg.1955]

Moorehead WR, Biggs HG. 2-Amino-2-methyl-1-propanol as the alkahzmg agent in an improved continuous-flow cresolphthalein complexone procedure for calcium in serum. Chn Chera 1974 20 1458-60. [Pg.1957]

Stern J, Lewis WHP. The colorimetric estimation of calcium in serum with o-cresolphthaiein complexone. Clin Chim Acta 1957 2 576-80. [Pg.1962]

T5. Trinder, P., Colorimetric micro-determination of calcium in serum. Analyst 86, 889-894 (1960). [Pg.155]

J. Ruzicka, J.C. Tjell, Ion-selective electrodes in continuous-flow analysis. Determination of calcium in serum, Anal. Chim. Acta 47 (1969) 475. [Pg.204]

Fig, 6. Relationship between calcium and phosphate concentrations in buffer solutions of different pH s in equilibrium with dead bone. The curves are based on the [Ca]3 [P]2 values shown in Fig. 5 and indicate that at any given phosphorus level a small change in bone pH causes a large change in calcium concentration. The ordinate on the right takes into account the protein-binding of calcium in serum and indicates the approximate equivalent calcium concentration if the in vitro observations were applicable in vivo. [Pg.287]

You agree to participate in a collaborative study for the determination of calcium in serum. A sample containing 5.2 meq/dL, with a standard deviation of 0.2 meq/dL, is sent to 10 laboratories for analysis using atomic absorption spectroscopy. You obtain triplicate results of 5.0, 4.7, and 4.8 meq/dL. What is the z value for your laboratory What do the results imply ... [Pg.134]

Serum. Calcium in serum or an artificial serum as described, in the footnote to Experiment 30 is determmed by diluting 1 20 with 1% SrCl2 solution. The normal calcium content of serum is about 100 ppm, and so that analyzed solution contains about 5 ppm Ca. Sodium and potassium equal to that in the sample are added to the standards. [Pg.777]

Heparinoids and mucopolysaccharides react with, and modify, many of the plasma proteins. Heparin combines with fibrinogen, globulins and albumin. As judged by electrophoresis and various types of analysis and staining, the particular plasma protein components with which heparin combines are dependent upon the concentration of protein, concentration of heparin, pH value, and salts present. This explains the somewhat contradictory statements in literature about combinations of heparin with plasma proteins. The combination may result in change of solubility of the protein and reverse protein tests . Heparin can modify the murexide reaction for calcium in serum by affecting the calcium-protein-heparin complex. Many heparinoids... [Pg.146]

FIGURE 61-1 Pools of calcium in serum. Concentrations are eiqiressed as mg/dL on the left-hand axis and as mM on the right. The total serum calcium concentration is 10 mg/dL or 2.5 mM, divided into three pools protein-bound (40%), complexed with small anions (10%), and ionized calcium (50%). The complexed and ionized pools represent die diffusible forms of calcium. [Pg.1060]

E. H. Hansen, J. ROiiCka, and A. K. Ghose, Flow Injection Analysis for Calcium in Serum, Water and Waste Waters by Spectrophotometry and by Ion-Selective Electrode. Anal. Chim. Acta, 100 (1978) 151. [Pg.384]

Brown, S.S., Healy, M.J.R. and Kearns, M. (1981a) Report on the interlaboratory trial of the reference method for the determination of total calcium in serum. Part I, J. Clin. Chem. Clin. Biochem. 19, 395. [Pg.228]

Medium dispersion corresponds to D values of 3 -10. I igure 33-8a illustrates a medium-dispersion system for the colorimetric determination of calcium in serum, milk, and drinking water. A borax buffer and a color reagent are combined in a 50-cm mixing coil A prior to sample injection. The output for three samples in iriplicate and four standards in duplicate is shown in Figure 33-8b. [Pg.936]

There are three forms of calcium in serum that can fluctuate among forms depending on changes to the serum pH and/or serum protein (albumin) levels. [Pg.195]

Determination of Magnesium and Calcium in Serum Ame Jensen and Erik Riber... [Pg.291]

Recommendations on the Reference Method for the Determination of Ionized Calcium in Serum, Plasma or Whole Blood ... [Pg.313]

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