Calcium ion in serum

A cahbration curve of potential versus log activity is usually prepared. If concentrations are to be measured, then the technique of maintaining a constant ionic strength as described earlier is used (Equation 13.34). For example, the concentration of unbound calcium ion in serum is determined by diluting samples and standards with 0.15 M NaCl. Only the unbound calcium is measured and not the fraction that is complexed. 

Munro et al. showed separation and detection of amino acids on microchips using an indirect fluorescence detection method. Figure 36.10 shows application of this method to urine samples with no pretreatment other than dilution in the appropriate separation buffer. Abnormal amounts of amino acids can easily be detected in the two patient samples compared to the healthy control sample. An absorbance detection based approach was utilized for the clinical analysis of calcium ion in serum, which is important in the regulation of a number of physiological processes. Beads with an immobilized calcium reactive dye were placed into the detection region, and the samples mobilized past the beads using electrophoretic flow. While a true separation was not intended, the interference... 

There has been some uncertainty concerning the concentration of free calcium ions in serum... 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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