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Briggs-Haldane constant

ICp, Kg Briggs-Haldane constants for fructose to glucose and glucose to fructose, i.e.,... [Pg.32]

A mathematical equation indicating how the equilibrium constant of an enzyme-catalyzed reaction (or half-reaction in the case of so-called ping pong reaction mechanisms) is related to the various kinetic parameters for the reaction mechanism. In the Briggs-Haldane steady-state treatment of a Uni Uni reaction mechanism, the Haldane relation can be written as follows ... [Pg.327]

In the Briggs-Haldane steady-state treatment of a one-substrate enzyme system, the Michaelis constant, usually symbolized by, is ( 2 + k3)/ki. For more complex reactions (e.g., with several substrates and/or isomerization steps), the Michaelis constant for a given substrate is a more complex collection of rate constants. For a multisubstrate enzyme having substrates A and B, the Michaelis constants are usually symbolized by and, by and, or by and, respectively. [Pg.466]

An enzyme is said to obey Michaelis-Menten kinetics, if a plot of the initial reaction rate (in which the substrate concentration is in great excess over the total enzyme concentration) versus substrate concentration(s) produces a hyperbolic curve. There should be no cooperativity apparent in the rate-saturation process, and the initial rate behavior should comply with the Michaelis-Menten equation, v = Emax[A]/(7 a + [A]), where v is the initial velocity, [A] is the initial substrate concentration, Umax is the maximum velocity, and is the dissociation constant for the substrate. A, binding to the free enzyme. The original formulation of the Michaelis-Menten treatment assumed a rapid pre-equilibrium of E and S with the central complex EX. However, the steady-state or Briggs-Haldane derivation yields an equation that is iso-... [Pg.467]

In the Briggs-Maldane mechanism, when k2 is much greater than k-i, kcJKM is equal to kx, the rate constant for the association of enzyme and substrate. It is shown in Chapter 4 that association rate constants should be on the order of 108 s l M l. This leads to a diagnostic test for the Briggs-Haldane mechanism the value of kaJKu is about 107 to 108 s-1 M-1. Catalase, acetylcholinesterase, carbonic anhydrase, crotonase, fumarase, and triosephosphate isomerase all exhibit Briggs-Haldane kinetics by this criterion (see Chapter 4, Table 4.4). [Pg.65]

Table 4.5 shows that for some efficient enzymes, kcJKM may be as high as 3 X 108 s-1 M l. In these cases, the rate-determining step for this parameter, which is the apparent second-order rate constant for the reaction of free enzyme with free substrate, is close to the diffusion-controlled encounter of the enzyme and the substrate. Briggs-Haldane kinetics holds for these enzymes (Chapter 3, section B3). [Pg.94]

As we discussed in Chapter 3, the KM for an enzymatic reaction is not always equal to the dissociation constant of the enzyme-substrate complex, but may be lower or higher depending on whether or not intermediates accumulate or Briggs-Haldane kinetics hold. Enzyme-substrate dissociation constants cannot be derived from steady state kinetics unless mechanistic assumptions are made or there is corroborative evidence. Pre-steady state kinetics are more powerful, since the chemical steps may often be separated from those for binding. [Pg.112]

One example in which specificity may be lost is when Briggs-Haldane kinetics are occurring (Chapter 3, section A3a). Under these conditions, kCdLl/KM is equal to the rate constant for the association of the enzyme and the substrate. Since it is usually found that the higher dissociation constants for smaller substrates arise from a higher rate of dissociation rather than from a lower rate of association, there will be a partial or complete loss of specificity. [Pg.201]

Dissociation rate constants are much lower than the diffusion-controlled limit, since the forces responsible for the binding must be overcome in the dissociation step. In some cases, enzyme-substrate dissociation is slower than the subsequent chemical steps, and this gives rise to Briggs-Haldane kinetics. [Pg.421]

Significance of the Specificity Constant, kcat/Km. Under physiological conditions, enzymes usually do not operate at saturating substrate concentrations. More typically, the ratio of the substrate concentration to the Km is in the range of 0.01-1.0. If [S] is much smaller than Km, the denominator of the Briggs-Haldane equation [equation (25)] is approximately equal to Km, so that the velocity of the reaction becomes... [Pg.144]

The one-plus rate equation 8.22 is of the same algebraic form as the Michaelis-Menten equation 8.18, only the physical significance of the coefficients is different [instead of the constant K, the expression kAX /(k + kXP) now appears]. Accordingly, the behavior is the same as for Michaelis-Menten kinetics, and that name is often used for Briggs-Haldane kinetics as well. [Pg.209]

As shown above, the equilibrium assumption leads to a version of the Michaelis-Menten equation in which the Michaelis constant is equivalent to the dissociation constant for the enzyme-substrate complex EA. This unfortunately encourages many biochemists to assume that Michaelis constants can always be so equated. The misleading statement that K, reflects an enzyme s affinity for its substrate is often encountered. Even if we consider only 1-substrate enzymes, the Briggs-Haldane version of will only approximate to k /k if However, if, for instance, /c2 = 10/c, then is 11 times greater than the dissociation constant for EA. If kj k, then = k2/k rather than k /k. ... [Pg.78]

In order to solve Equation (8.3), expressions for [E] and [ES] are required that are obtained by applying Briggs-Haldane steady state principles. According to these principles, biocatalysis rapidly attains a condition of stasis under which all biocatalyst species are at a constant equilibrium concentration. In other words [E] and [ES] are constant with time. Stasis is reflected by... [Pg.409]

The corresponding kinetic scheme is as illustrated in Scheme 8.21. This kinetic scheme bears some similarity to the simplest kinetic scheme and hence the simplest Briggs-Haldane steady state kinetics treatment can usefully apply on the assumption that the donor species D is in excess (i.e., [D] 2> [A]) and so is constant during the progress of the reaction. In this case, we can make the assumption that acceptor species A behaves in an equivalent manner to a biocatalyst substrate and donor species D to the biocatalyst itself at a fixed total concentration of [D]q. Hence, Equation (8.6) neatly transforms into... [Pg.471]

Thus, the observed rate constant depends on the substrate concentration and on the three fundamental rate eonstants. It is obviously of the same form as the Briggs-Haldane treatment for a one-substrate enzyme reaction (steady-state treatment, see also eqn (4.2b)). A detailed derivation utilized by Das et al. can be found in the supporting information of ref. 41. Unfortunately this function can only lead to approximated values, which is mainly caused by... [Pg.127]

The rate coefficients can now be combined into the Michaelis constant Ku (hi the Briggs-Haldane version) which is a quantity characteristic of a given enzyme and given substrate,... [Pg.462]

It is important to note that the derivation of the rate law in the Briggs-Haldane mechanism gives the same result as in the Michaelis-Menten mechanism, namely the fundamental Michaehs-Menten equation (3.9). However, in the former case, the Michaelis constant Ka is increased by a factor k /k, compared with the latter case Vmax constant has the same meaning in both mechanisms. [Pg.36]

At low substrate concentrations, the enzyme is largely unbound and E Eo therefore, fccat/ A is an apparent second-order rate constant, which is not a tme microscopic rate constant except in the extreme case in which the rate-limiting step in the reaction is the encounter of enzyme and substrate. Only in the Briggs-Haldane mechanism, when is much greater than fc, fccat/JSC is equal to ku the rate constant for the association of enzyme and substrate. Recently, Northrop (1999) raised a serious objection to this classical definition of the specificity constant, and pointed out that fcc t/ 0 actually provides a measure of the rate of capture of substrate by free enzyme into a productive complex or complexes destined to go on to form products and complete a turnover at some later time. [Pg.44]

Perusal of the physicochemical chapters of textbooks on physiology and biochemistry, published up to the 1950s, reveals an overwhelming concern with the analysis of equilibria. This interest in the presentation of detailed and useful accounts of ionic processes and the energy balance of metabolic pathways left little space for attention to rate processes. Briggs Haldane (1925) introduced the steady state treatment of simple enzyme reactions, as opposed to the earlier, unrealistic, equilibrium approach (see section 3.3). Since then, and especially from the 1950s onwards, there has been more appreciation of the fact that cellular processes are in a constant state of flux or are in a steady state. Individual reactions may be at or near equilibrium, but for the cell as a whole equilibrium is death. [Pg.107]

The interpretations of Michaelis and Menten were refined and extended in 1925 by Briggs and Haldane, by assuming the concentration of the enzyme-substrate complex ES quickly reaches a constant value in such a dynamic system. That is, ES is formed as rapidly from E + S as it disappears by its two possible fates dissociation to regenerate E + S, and reaction to form E + P. This assumption is termed the steady-state assumption and is expressed as... [Pg.435]

In Equation 11.9 we reserve the missing rate constant k4 for an elaboration of the mechanism). Following Briggs and Haldane we make the assumption that the steady-state approximation applies to ES and EP complexes ... [Pg.347]

When the enzyme is first mixed with a large excess of substrate, there is an initial period, the pre-steady state, during which the concentration of ES builds up. This period is usually too short to be easily observed, lasting just microseconds. The reaction quickly achieves a steady state in which [ES] (and the concentrations of any other intermediates) remains approximately constant over time. The concept of a steady state was introduced by G. E. Briggs and Haldane in 1925. The measured V0 generally reflects the steady state, even though V0 is limited to the early part of the reaction, and analysis of these initial rates is referred to as steady-state kinetics. [Pg.203]

Briggs and Haldane [8] proposed a general mathematical description of enzymatic kinetic reaction. Their model is based on the assumption that after a short initial startup period, the concentration of the enzyme-substrate complex is in a pseudo-steady state (PSS). For a constant volume batch reactor operated at constant temperature T, and pH, the rate expressions and material balances on S, E, ES, and P are... [Pg.840]


See other pages where Briggs-Haldane constant is mentioned: [Pg.393]    [Pg.100]    [Pg.427]    [Pg.426]    [Pg.179]    [Pg.1515]    [Pg.7]    [Pg.571]    [Pg.406]    [Pg.28]    [Pg.52]    [Pg.142]    [Pg.426]    [Pg.111]    [Pg.142]    [Pg.10]   
See also in sourсe #XX -- [ Pg.336 ]

See also in sourсe #XX -- [ Pg.301 ]




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