Brevibacterium lactofermentum

Glucose, Sucrose, Fructose Pseudomonas fluorescens, Bacillus subtilus, Brevibacterium lactofermentum 71, 72,73... 

In the best-documented example of the formation of lysine, the product is formed from aspartate which reacts via aspartylphosphate and aspartate semialdehyde to lysine. The wild type of Brevibacterium lactofermentum does not produce any lysine. With the following steps the yield could be increased to 50 g IT1 ... 

L-Leucine Glucose Brevibacterium lactofermentum Serratia marcescens... 

Assimilable sugars Brevibacterium lactofermentum Hikuma et al. (1980a)... 

L-Lysine is produced by some mutants induced from wild strain of glutamate-producing bacteria including Corynebacterium glutamicum, Brevibacterium lactofermentum, and B. flavum in the presence of high concentrations of sugar and ammonium ions at neutral pH and under aerobic condition [2]. 

L-Threonine is produced by some auxotrophic mutants and/or threonine-analog resistant mutants and those bred by gene engineering techniques. The bacteria are Escherichia coli, Corynebacterium glutamicum, Brevibacterium lactofermentum, B.flavum, Serratia marcescens, and Proteus retgerii. 

Sano, K., Ito, K., Miwa, K., and Nakamori, S, (1987) Amplification of the phosphoenol pyruvate carboxylase gene of Brevibacterium lactofermentum to improve amino acid production. Agric. Biol Chem., 51 (2), 597-599. 

Haynes, J.A. and Britz, M.L. (1989) Electro transformation of Brevibacterium lactofermentum and Corynebacterium glutamicum. growth in tween 80 increases transformation frequencies. FEMS Microbiol Lett, 61 (3 329-334. 

Adham, S.A., Campelo, A.B., Ramos, A., and Gil, J.A. (2001) Construction of a xylanase-producing strain of Brevibacterium lactofermentum by stable integration of an engineered xysA gene from Streptomyces halstedii JM8. Appl. Environ. Microbiol, 67 (12), 5425 - 5430. 

Kawahara Y, Takahashi-Fuke K, Shimizu E, Nakamatsu T, Nakamori S. (1997). Relationship between the glutamate production and the activity of 2-oxoglutarate dehydrogenase in Brevibacterium lactofermentum. Biosci Biotechnol Biochem, 61, 1109-1112. 

Takinami K, Yoshii H, Tsuri H, Okada H. (1965). Biochemical effects of fatty acid and its derivatives on L-glutamie aeid fermentation. Part III. Biotin-Tween 60 relationship in the accumulation of L-glutamic acid and the growth of Brevibacterium lactofermentum. Agric Biol Chem, 29, 351-359. 

Kimura E, Abe C, KavraharaY, Nakamatsu T. Molecular cloning of a novel gene, derived from Brevibacterium lactofermentum. Biosci Biotechnol Biochem 1996 60 1565-70. 

Kimura E, Abe C, Kawahara Y, Nakamatsu T,Tokuda H. A dtsR gene-disrupted mutant of Brevibacterium lactofermentum requires fatty acids for growth and efficiently produces L-glutamate in the presence... 

Morinage Y, Takagi H, Ishida M, Miwa K, Sato T, Nakamori S, et al. Threonine production by coexistence of cloned genes coding homoserine dehydrogenase and homoserine kinase in Brevibacterium lactofermentum. Agric Biol Chem 1987 51 93-100. 

Mateos LM, Pisabarro A, Patek M, Malumbres M, Guerrero C, Eikmanns BJ, Sahm H, Martin JF (1994) Transcriptional analysis and regulatory signals ofthe. hom-thrB cluster of Brevibacterium lactofermentum. J Bacteriol 176 7362-7371... 

L-Phenylalanine. L-phenylalanine is produced via fermentation using a mutant Brevibacterium lactofermentum 2256 (ATCC No. 13869) known as No. 123 [2]. The rate equations for biomass (bacteria, X), substrate (mainly glucose, S), and product (L-phenylalanine, P) are described by Monod kinetics. 

Table B.8.1 Best-Fit Parameters for Monod Kinetics Usii Brevibacterium lactofermentum...

Liebl, W., Ehrmann, M., Ludwig, W, and Schleifer, K.H. (1991) Transfer of Bre-vibacterium divaricatum DSM 20297T, "Brevibacterium flavum" DSM 20411, "Brevibacterium lactofermentum" DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction... 

L-Threonine is produced by some auxotrophic mutants or threonine-analogue-resistant mutants, and those are created by genetic engineering techniques. The bacteria used are Escherichia coli, Corynebacterium glutamicum, Brevibacterium lactofermentum, B. flavum, Serratia marcescens, and Proteus rettgeri (Nakamori 1986). L-Threonine production by fermentation was started in the 1970s. The auxotrophic mutant and analogue-resistant mutant strains obtained for this purpose were cultured in the presence of amino acids required by the mutant. 

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