Bovine brain ganglioside

Since interferons appear to be species-specific, we investigated whether the ganglioside fraction from mouse brain was more potent in inhibiting antiviral activity of mouse fibroblast interferon than that obtained from heterologous brain extracts. As seen in Figure 1 bovine brain gangliosides were almost as potent... 

Cells were grown in RPMI medium plus 5 % fetal bovine serum in 1 mL total volume as described in Materials and Methods. At 24-hr intervals the cells were counted in a Coulter counter. Control cells 0, cells from cultures containing 1000 lU/mL mouse fibroblast interferon , cells from cultures containing bovine brain gangliosides at a concentration corresponding to 35 p.M sialic acid , cells from cultures containing both interferon (1000 IU/mL) and gangliosides (35 p.M sialic acid). 

We also investigated the specificities of purified antibodies against G i and Gp, from the serum of a rabbit immunized with bovine brain gangliosides. As shown in Figure 6, the whole antiserum had substantial activity both against the two ganglio-... 

Figure 6. Reactivities of whole antiserum and purified anti-Gll and anti-Gn,b antibodies against liposomes containing glycolipids. Antibodies purified from rabbit anti-bovine brain ganglioside serum as described previously (12). Glucose release measured from liposomes containing DMPC, CHOL, DCP, and either Cm, Gpn CMH, CDH, DDG (each 150 p.g/ymol PC) or CTH (150 nmol/pmol PC).

Fibronectin mediates the adhesion of cells to collagen by first binding to the collagen substrate followed by attachment of the cells to the fibronectin-collagen complex. Bovine brain gangliosides prevented the adhesion of cells to the fibronectin-collagen complex, but had no effect on the initial formation of the fibronectin-collagen complex. The order of efficiency was and G > Gd, j Gj > was not an inhibitor. Only the carbohydrate portions of... 

Bovine brain gangliosides were oxidized by periodate ion and iV -2,4-dinitro-phenyl-L-2,4-diaminobutyric acid hydrazide was covalently attached to the aldehyde groups. The dinitrophenyl-labelled gangliosides were incorporated into mature thymocytes and these were incubated with rabbit anti-dinitrophenol and fluorescein-labelled anti-rabbit immunoglobulin G, causing uniform rings on the cell surface. Gangliosides may play a primary role in the lymphocyte activation process. 

Ultracentrifugation has shown that changes in the content of either sialic acid or C2o sphingosine affect the size of the micelles formed by bovine-brain gangliosides-increases in the content of sialic acid decreasing the size of the micelle. The gangliosides from bovine brain possess physical properties that differ from... 

Bovine brain Lysosomal-mitochondrial Bovine brain gangliosides 4.3 Gielen and Harprecht (1969)... 

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