Bovine, biological effects

FRET to occur, and thus no FI emission is observed. The specificity of this assay was also examined for mixed samples. The mixed lysozyme samples were prepared in fetal bovine serum (FBS), human saliva and human urine. It was found that FAM emission was still visible upon addition of each mixed sample, implying that this assay has a great potential for the detection of real biological samples. This study illuminates that introduction of specific aptamer/protein interaction as the recognition event, and utilization of FRET as the signal transduction channel, is an effective way to develop CPE-based protein sensors with good specificity. 

Vandenbroeck et al.7 used an ELISA to determine the recovery of immu-noreactive porcine interferon-gamma (IFN-y) from E. coli inclusion bodies. The ELISA used a polyclonal coating antibody with detection by a MAb. The inclusion bodies were solubilized in diluted 6 M guanidine/HCl and IFN subsequently refolded by its removal. The antiviral activity of the interferon was measured with a bioassay using the cytopathic effect (CPE) of vesicular stomatitis virus (VSV) on bovine kidney cells. The results of this study showed that the immu-noreactivity measured by ELISA matched the biological activity measured by bioassay. 

Urea in kidney dialysate can be determined by immobilizing urease (via silylation or with glutaraldehyde as binder) on commercially available acid-base cellulose pads the process has to be modified slightly in order not to alter the dye contained in the pads [57]. The stopped-flow technique assures the required sensitivity for the enzymatic reaction, which takes 30-60 s. Synchronization of the peristaltic pumps PI and P2 in the valveless impulse-response flow injection manifold depicted in Fig. 5.19.B by means of a timer enables kinetic measurements [62]. Following a comprehensive study of the effect of hydrodynamic and (bio)chemical variables, the sensor was optimized for monitoring urea in real biological samples. A similar system was used for the determination of penicillin by penicillinase-catalysed hydrolysis. The enzyme was immobilized on acid-base cellulose strips via bovine serum albumin similarly as in enzyme electrodes [63], even though the above-described procedure would have been equally effective. 

Neurological Effects. Little information was available to determine the neurotoxicity or the mechanism of neurotoxicity of HDI after inhalation, oral, or dermal exposure. Headache was reported in only one human exposure case (Malo et al. 1983). Neurotoxic effects (convulsions) may occur in laboratory animals if concentrations reaeh high levels in the air (Haskell Laboratory 1961) however, sinee HDI is metabolized quickly in a biological matrix (Berode et al. 1991), little intaet HDI is expected to reach the nervous tissue to elicit a toxic response, except possibly at very high eoneentrations. No neurological effects have reported in laboratory animals, or in hiunans exposed chronieally to low concentrations of HDI (Mobay Corporation 1989). HDI, in addition to other isocyanates, have been shown to inhibit acetylcholinesterase in human erythrocytes (Dewair et al. 1983), human serum acetylcholinesterase (Brown et al. 1982), as well as equine serum, bovine erythrocyte, and eel acetylcholinesterase (Brown et al. 1982). 

Chrysina, E.D., Brew, K., Acharya, K.R. (2000). Crystal structure ofapo- and liolo-bovine a-lactalbumin at 2.2-A resolution reveals an effect of calcium on inter-lobe interactions. Journal of Biological Chemistry, 275, 37021-37029. 

In 1975, the Life Sciences Research Office of the Federation of American Societies for Experimental Biology (Carr et al. 1975), upon an extensive review of the available evidence, concluded that it was doubtful whether XO in homogenized cow s milk was a causal or risk factor for heart disease. More recently, Clifford et al (1983) and Deeth (1983), in critical reviews of the homogenized cow s milk XO hypothesis, have arrived at a similar, if not more definitive, conclusion. As stated by Clifford et al. (1983), experimental evidence has failed to substantiate, and in many cases has refuted, the hypothesis that homogenized bovine milk xanthine oxidase intake or plasmalogen depletion are causal factors in the development of atherosclerosis. And, according to Deeth (1983), there appears to be no unequivocal evidence that the absorbed enzyme has any pathological effects that may contribute to development of atherosclerotic heart disease. ... 

For many years patients with diabetes were treated with insulins that had been isolated from the pancreases of pigs and cows. The primary sequences of these insulins are closely related to the sequence of human insulin. There is only a single difference between the sequences of human and porcine insulins human insulin has a threonine at position B30, and porcine insulin has an alanine. Bovine insulin differs from human insulin at three positions. There is an alanine at the B30 position, an alanine at A8, and a valine at A10. These conservative changes in primary sequence have no apparent effect on biologic activity, although there are slight differences in solubility (7). 

Because of the ease with which molecular mechanics calculations may be obtained, there was early recognition that inclusion of solvation effects, particularly for biological molecules associated with water, was essential to describe experimentally observed structures and phenomena [32]. The solvent, usually an aqueous phase, has a fundamental influence on the structure, thermodynamics, and dynamics of proteins at both a global and local level [3/]. Inclusion of solvent effects in a simulation of bovine pancreatic trypsin inhibitor produced a time-averaged structure much more like that observed in high-resolution X-ray studies with smaller atomic amplitudes of vibration and a fewer number of incorrect hydrogen bonds [33], High-resolution proton NMR studies of protein hydration in aqueous... 

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