Boron boronate affinity

Crude chloroform-methanol-water (30 60 8, v/v) extracts of immunostainedTLC bands were analyzed without further purification by nanoelectrospray low-energy mass spectrometry. The authors showed that this effective PLC/MS-joined procedure offers a wide range of applications for any carbohydrate-binding agents such as bacterial toxins, plant lectins, and others. Phenyl-boronic acid (PBA) immobilized on stationary support phases can be put to similar applications. This technology, named boronate affinity chromatography (BAC), consists of a chemical reaction of 1,2- and 1,3-diols with the bonded-phase PBA to form a stable... 

Boron-aluminum-magnesium alloys, 4 137 Boron analysis, of water, 26 39 Boronate affinity chromatography, 6 403 04... 

Cook, N. D. and Peters, T. J. (1985) Purification of y-glutamyl transferase by phenyl boronate affinity chromatography. Biochim. Biophys. Acta 828,205—212. 

Boronate affinity Sugar complex All antibodies Low specificity... 

Cook N D and Peters T J (1985) Punfication of yglutamyl transferase by phenyl boronate affinity chromatography Biochim Bwphys. ActaSiS, 2.06-11%... 

Boronic acid and its derivatives are another class of nonbiological ligands that have weak binding and are often used under isocratic conditions. These are employed in the method of boronate affinity chromatography, which makes use of the ability of such ligands to form covalent... 

Tanaka T, Matsunaga T (2001) Detection of HbA(lc) by boronate affinity immunoassay using bacterial magnetic particles. Biosens Bioelectron 16 1089-1094... 

Dihydroxyphenylalanine-containing proteins. Boronate affinity chromatography is used in the selective binding of proteins containing 3,4-dihydroxyphenylalanine to a m-phenylboronate agarose column and eluted with IM NH4OAC at pH 10. [Hankus et al. Anal Biochem 150 187 7956.]... 

Fig. 10. Glycated Cu,Zn-SOD in streptozotocin-induced diabetic rat lens. Pooled lenses from normal and streptozotocin-induced diabetic rats [9 weeks after intravenous injection of streptozotocin (STZ)] were homogenized, and Cu,Zn-SOD was extracted by chloroform/ethanol and subjected to a boronate affinity column. Upper panel, normal rat lens lower panel, diabetic rat lens. The SOD activity and immunoreactive Cu,Zn-SOD were measured using the xanthine/xanthine oxidase method and ELISA, respectively.

Quantitate Bond Elut Boronate affinity column... 

Figure 2. Ve have demonstrated the quantitative recovery of thymidine by boronate affinity chromatography and the quantitative retention of adenosine with up to 1 ymole quantities.

A polyacrylamide-boronate affinity gel, Affi-gel 601 , has been used for the separation of cyclic adenosine monophosphate, cyclic guanosine monophosphate, and cyclic cytidine monophosphate from their corresponding 5-nucleotides and nucleosides. A simple direct assay of 3 5 -cyclic nucleotide phosphodiesterase activity has been developed, based on the use of the gel. 

Zhang, Q.B. Tang, N. Brock, J.W.C. Mottaz, H.M. Ames, J.M. Baynes, J.W. Smith, R.D. Metz, TO. Enrichment and analysis of nonenzymatically glycated peptides Boronate affinity chromatography coupled with electron-transfer dissociation mass spectrometry. J. Proteome Res. 2007, 6, 2323-2330. 

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