Sugar affinated

Affioade, /. washed raw sugar, affinShnlich, a. similar in affinity. 

Acetyl esterase (AE) has been purified to homogeneity from orange peels. The purification steps included cation exchange chromatography and gel filtration. The enzyme has affinity for triacetin and sugar beet pectin with K, of 39 mM and of 26 mg/ml, respectively. AE has a MW of 42 kD and is a monomer. The isoelectric point is at pH > 9. 

The affinity for sugar beet pectin was determined using a Lineweaver-Burk plot. The K was calculated to be 26 mg/ml for sugar beet pectin whereas the Kj, for triacetin was 39 mM. This showed a very low affinity for sugar beet pectin and triacetin. Substrate specifity of purified AE is summarized in table 2. 

In addition, the data indicated that the strong inhibition = 4/iM) of the exchange reaction by the unphosphorylated sugar, a phenomenon reported for many E-IIs [73,104-106] was due to competition between fructose and fructose-l-P for the binding site on the enzyme. The important implication of this result was that it would indicate that unphosphorylated II possessed a high affinity for the sugar, which, subsequently, was confirmed by binding studies. Furthermore, it was concluded that... 

Yeasts contain a large number of different active and passive sugar-transport systems. The first of these to be cloned was the glucose-repressible, high-affinity passive glucose transporter of Saccharomyces cerevisiae, which is encoded by the SNF3 gene... 

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