3,4-Dihydroxyphenylalanine-containing proteins

Dihydroxyphenylalanine-containing proteins. Boronate affinity chromatography is used in the selective binding of proteins containing 3,4-dihydroxyphenylalanine to a m-phenylboronate agarose column and eluted with IM NH4OAC at pH 10. [Hankus et al. Anal Biochem 150 187 7956.]... 

Taylor, S.W., Luther 111, G.W., Waite, J.H. Polarographic and spectrophotometric investigation of iron (111) complexation to 3,4-dihydroxyphenylalanine-containing peptides and proteins from Mytilus edulis. Inorg. Chem. 33(25), 5819-5824 (1994)... 

The 216-residue hen egg yolk storage protein phosvitin contains 123 serine residues, most of which have been phosphorylated (Eq. 2-16).295 A basic protein of the myelin sheath of neurons contains as many as 6 specific residues of citrulline (Eq. 2-19).296 An adhesive protein from the foot of a marine mollusk contains -80 repeated sequences containing hydroxy-proline 2,3-dihydroxyproline and 3,4-dihydroxyphenylalanine (Dopa) 297/298... 

In addition to rubber, latex contains 0.3-0.7% ash, 1-2% nitrogenous compounds, 2% resins, and 1-2% sugars and related compounds. The proteins represent most of the nitrogenous compounds in the latex and upon hydrolysis yield alanine, aspartic acid, dihydroxyphenylalanine, glutamic acid, histidine, leucine, ornithine, hydroxyproline, tyrosine, and valine. Lecithin, glycerophosphoric acid, fatty acids (palmitic, stearic, arachidic, oleic, and linoleic), sterols, resin acids, wax, and quebrachitol are some of the other nonrubber constituents. The mineral constituents present in the ash consist of potassium, magnesium with traces of copper, iron, and manganese. 

Dorset , L.C., Hawkins, C.J., Grice, J.A., Lavin, M.F., Merefield, P.M., Parry, D.L., and Ross, I.L. (1987) Ferrasddin a highly aromatic protein containing 3,4-dihydroxyphenylalanine from the blood cells of a stohdobranch ascidian. Biochemistry, 26, 8078—8082. 

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