BLUE TETRAZOLIUM BT

Physical Form Lemon yellow crystals or powder 

Solubility Soluble in water freely soluble ethanol, methanol, chloroform insoluble in acetone, ether, ethyl acetate 

CA Index Name 2 -Tetraolium, 2,2 -(3,3 -dimethoxy [l,l -biphenyl]-4,4 -diyl)bis[3,5-diphenyl-, chloride (1 2) 

2//-Tetrazolium,3,3 -(3,3 -dimethoxy-4,4 -biphenylylene) his[2,5-diphenyl-, dichloride 3,3 -(3,3 -Dimethoxy-4,4 -hiphenylylene)his [2,5 -diphenyl-2H-tetrazolium chlo- 

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Blue tetrazolium (BT) and Phenylhydrazine H2SO4 (PH) reagents react with the intact side chain at C17 of prednisolone while isonicotinic acid hydrazide (INH) and UV-absorption depend upon conjugation in Ring A at the other end of the prednisolone molecule. Since... 

Neither 21-hydroxylation, ring A or 20-keto reduction is inhibited by Acutely hypoxed rats secrete more blue tetrazolium reducing (BT+) steroids following M suggesting a direct stimulation of the adrenal 3. However, rats secrete a BT- steroid, 18-OH DOC, and thus inhibition of 11- and 18-hydroxylations by M could result in an amount of BT+ DOC equal to B +18-0H DOC. While total BT+ material would increase, the total amount of steroid could be the same and a direct effect on the adrenal need not be considered. 

Nitro BT. [Monomer-Polymer Dajac Labs] Nitro blue tetrazolium. 

EINECS 206-0674 NBT NBT (dye) Nitro Blue tetrazolium salt Nitro Blue tetrazolium chloride Nitro BT Nitro tetrazolium BT Nitroblue tetrazolium Nitroblue tetrazolium salt (4-Nitrophenyl)-5-phenyl-,dichloride Nitrotetrazolium Blue Nitrotetrazolium Chloride Blue NSC 27622 p-Nitro Blue tetrazolium p-Nitro Blue tetrazolium chloride p-Nitrotetrazolium Blue 2H-Tetrazolium, 3,3 -(3,3 -dimethoxy(1,1 -biphenyl)4,4 -diyl)bis(24,4 -ylene)ditetrazolium dichloride Tetraz-olium nitro BT Tetrazolium Nitro Blue. Activity slain for electrophoresis and histochemistry. Substrate for dehydrogenase. Crystals mp = 200". Monomer-Polymer Dajac. 

A 50892 4-Anisyltetrazollum blue Blue tetrazolium Blue tetrazolium chloride BT (VAN), 3,3 -Dianisole-bis(4,4 -(3,6-diphenyl)tetrazoliumchloride) 3,3 -(3,3 -Di-methoxy-4,4 -... 

Other Names 2//-Tetrazolium, 2,2 -(3,3 -dimethoxy [l,l -biphenyl]-4,4 -diyl)bis[3-(4-nitrophenyl)-5-phe-nyl]-, dichloride 27/-Tetrazolium, 3,3 -(3,3 -dimethoxy-4,4 -biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-, dichloride Tetrazolium, 3,3 -(3,3 -dimethoxy-4,4 -biphe-nylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-chloride] 2, 2 -Bis(p-nitrophenyl)-5,5 -diphenyl-3,3 -(3,3 -dimethoxy-4,4 -biphenylylene)ditetrazolium chloride 2,2 -Bis(p-ni-trophenyl)-5,5 -diphenyl-3,3 -(3,3 -dimethoxy-4,4 -di-phenylene)ditetrazolium chloride 2,2 -Di-p-nitrophenyl-5,5 -diphenyl-3,3 -bis(3,3 -dimethoxy-4,4 -biphenylene) ditetrazolium chloride 2,2 -Dinitrophenyl-5,5 -diphenyl-3,3 -dimethoxy-4,4 -diphenylene)ditetrazolium chloride 3,3 -(3,3 -Dimethoxy-4,4 -biphenylylene)bis[2-(p-nitro-phenyl)-5-phenyl-2H-tetrazolium chloride] 3,3 -(3,3 -Dimethoxy-4,4 -diphenylene)bis[2-(p-nitrophenyl)-5-phenyltetrazolium chloride] NBT NBT (dye) NSC 27622 NTB Nitro BT Nitro Blue Tetrazolium Nitro Blue Tetrazolium chloride Nitro Tetrazolium BT Nitro-tetrazolium Chloride Blue Nitrotetrazolium blue Tetrazolium Nitro BT Tetrazolium nitro blue /)-NBT />-Nitro blue tetrazolium /)-Nitro blue tetrazolium chloride p-Nitrotetrazolium blue... 

Fe reduction. Sections of roots can then be viewed under the microscope and the stain located. Bell et al. (1988) have evaluated the use of ferricyanide and the use of nitro-BT, [2,2 -di-/>-nitro-phenyl-5,5 -diphenyl]-3,3 -(3,3 -dimethoxy-4,4 -biphenylene)-di-tetrazolium chloride. With ferricyanide, iron-stressed tomato plants were mainly stained on the younger roots hairs located on the laterals or primary root tip. Nitro-BT is thought to compete with Fe(III) at the same transmembrane ferric reduction site (Sijmons and Bienfate, 1983). A purple diformazan precipitate is produced on reduction. Although the roots were stained in a similar way to those incubated with ferricyanide, Bell et al. (1988) point out that further research is required to determine if nitro-BT reduction completes with ferric reduction at the same site in the tomato. The iron-stress redox activity has been shown to be localised on the plasma membrane in tomato roots (Buckout et al., 1989), and electron microscopic examination (see next section) of the roots stained with Prussian blue indicated that the PB had accumulated between the plasma membrane and the cell walls of the root hairs and epidermal cells (Wergin et al., 1988). 

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