Nitro blue tetrazolium salt

Agar overlay with tetrazolium salt a-Naphthol plus dimethylpapraphenylenediamine a-Naphthylbutyrate plus diazo salt of fast blue RR Reduction of nitro blue tetrazolium salt Sodium a-naphthyl acid phosphate plus diazo salt of 5-chloro-o-toluidine... 

Reduction of silver phosphate to Ag by UV light Reduction of nitro blue tetrazolium salt... 

EINECS 206-0674 NBT NBT (dye) Nitro Blue tetrazolium salt Nitro Blue tetrazolium chloride Nitro BT Nitro tetrazolium BT Nitroblue tetrazolium Nitroblue tetrazolium salt (4-Nitrophenyl)-5-phenyl-,dichloride Nitrotetrazolium Blue Nitrotetrazolium Chloride Blue NSC 27622 p-Nitro Blue tetrazolium p-Nitro Blue tetrazolium chloride p-Nitrotetrazolium Blue 2H-Tetrazolium, 3,3 -(3,3 -dimethoxy(1,1 -biphenyl)4,4 -diyl)bis(24,4 -ylene)ditetrazolium dichloride Tetraz-olium nitro BT Tetrazolium Nitro Blue. Activity slain for electrophoresis and histochemistry. Substrate for dehydrogenase. Crystals mp = 200". Monomer-Polymer Dajac. 

APT buffer 100 mM Nad, 100 mM Tiis-Hd, pH9.5,50 mM Mgd, 1% Tween-20. NBT/BCIP staining mix Just before use, add 4.5 pL stock nitro blue tetrazolium salt (NBT) and 3.5 pL stock 5-bromo-4-chloro-3-indolyl phosphate (BCIP) to 1 mL APT buffer containing 2 mM levamisole. Stock NBT is 75 mg/mL in 70% dimethyl formamide, and stock BCIP is 50 mg/mL in 100% dimethyl formamide both stocks are supplied by Gibco-BRL as Immunoselect kit. 

List of Abbreviations BCIP, 5-bromo-4-chloro-3-indolyl phosphate, toluidine salt CCD, charged-coupled device CNS, central nervous system cDNA, copy or complimentary RNA cRNA, copy or complimentary RNA DNase, deoxyribonuclease FITC, fluorescein isothiocyanate mRNA, messenger RNA NBT, nitro blue tetrazolium choride PCR, polymerase chain reaction RNase, ribonclease... 

Prepare the color development solution by adding the BCIP [25 mg of 5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt (Sigma Chemical Co.) in 2.5 mL of N,N -dimethylformamide] and NBT (50 mg of nitro blue tetrazolium in 5 mL of 50% N, N -dimethylformamide) solutions to 100 mL of 1.0 M Tris-HCl, pH 9.0. 

Histochemistry Several NADPH consuming enzymes including NOS show an activity similar to NADPH diaphorase. The latter uses NADPH to convert soluble tetrazolium salts into insoluble visible formazan. Nitro blue tetrazolium is a widely used blue stain for NADPH diaphorase activity, and it has been observed that staining correlates well with NOS activity. The NOS contribution to the staining cannot be quantified, as several enzymes show diaphorase activity. 

Dye oxidation (e.g., tetrazolium reductase activity with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, MTT 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt, WST-1 3- (4,5 -carboxymethoxyphenyl) -2-(4-sulfophenyl)-2 H-tetra-zolium, MTS 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, XTT 2,2 -di-p-nitrophenyl-5,5 -diphenyl-3,3 -(3,3 -dimethoxy-4,4 -diphe-nylenej-ditetrazolium chloride, NET), Alamar blue assays, ATP concentration (e.g., luciferase assay), oxygen consumption (e.g., oxygen electrodes, phosphorescent oxygen-sensitive dyes), mitochondrial protein and nucleic acid synthesis mitochondrial mass (e.g., mitotracker dyes) mitochondrial membrane potential (e.g., tetramethylrho-damine methyl ester, TMRM tetramethylrhodamine ethyl ester, TMRE)... 

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