Bioscavengers

Doctor BP, Saxena A (2005) Bioscavengers for the protection of humans against organophosphate toxicity. Chem-Biol Interact 157-158 167-171... 

The result of the inherent comparison of AChE and BChE that was completed in our laboratory revealed two very adept OP bioscavengers. The major difference noted in this study was an ineffectiveness of oxime-mediated reactivation of BChE relative to the accelerated rates seen with AChE (Geyer, Cerasoli, Lenz, and Mor, unpublished results). In the case of both enzymes, by utilizing purification protocols that were only mildly adapted from those used to purify serum BChE or recombinant ChEs we can assume that any associated purification costs will be similar to those incurred in other systems. Before this project is ready to transition into a clinical study, a further refinement of the PEGylation and other modifications that have been shown to enhance the circulatory profile of these plant-derived ChEs must be undertaken. 

Doctor, B.P., Maxwell, D.M., Saxena, A. (1997). Preparation and characterization of bioscavengers for possible use against organophosphate toxicity. m-CB Medical Treatment Symposium, May 26-30, 1997, Hradec Kralove. Abstracts, pp. 17-18. 

Maxwell, D.M., Brecht, K., Saxena, A., Feaster, S., Doctor, B.P. (1998). Comparison of cholinesterases and carboxylesterase as bioscavengers for organophosphorus compounds. In Structure and Function of Cholinesterases and Related Proteins (B.P. Doctor et ah, eds), pp. 387-92. Plenum Press, New York. 

Moore, D.H. (1996). Bioscavengers as antidotes for organophosphorus (OP) agents. In Proceedings of the 2nd CB Medical Treatment Symposium, July 7-12, 1996, pp. 330 9. Spiez, Switzerland. 

Curtin, B.F., Seetharam, K.I., Dhoieam, P., Gordon, R.K., Doctor, B.P., Nambiar, M.P. (2008). Resveratrol induces catalytic bioscavenger paraoxonase 1 expression and protects against chemical warfare nerve agent toxicity in human cell lines. J. Cell. Biochem. 103 1054-35. 

Protection of Acetylcholinesterase from Organophosphates Kinetic Insight into Bioscavengers... 

TABLE 69.2. Substrate profile of enzymes with potential use as protective bioscavengers... 

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