Bioscavengers butyrylcholinesterase

Grunwald, J., Marcus, D., Papier, Y., Raveh, L., Pittel, Z. and Ashani, Y. (1997) Large-scale purification and long-term stability of human butyrylcholinesterase a potential bioscavenger drug. J Biochem Biophys Methods, 34, 123-35. 

Fast analysis of ofloxacin and lidocaine (as bactericide and analgesic) is of clinic importance for understanding the patient s medical process. A sensitive method for the determination of lidocaine, ofloxacin [46], enrolloxacin (ENR), and ciprofloxacin (CIP) [47] by CE integrated with ECL detection has been developed based on porous etched joint and end-column Ru(bpy)3 ECL and successfully applied to determine ENR and CIP in milk with a solid-phase extraction procedure. The proposed method explores detection limits of lidocaine, ofloxacin, enrofloxacin, and ciprofloxacin as 3.0 x 10 , 5.0 x 10 , 10 x 10 , and 15 x 10 mol L respectively (S/N = 3). CE-ECL detection method has also been used to characterize disopyramide with a detection limit of 2.5 x 10 mol L (S/N = 3) [48], and procaine hydrolysis as a probe for butyrylcholinesterase by in vitro procaine metabolism in plasma with butyrylcholinesterase acting as bioscavenger. Procaine and its metabolite N, A-diethylethanolamine were separated at 16 kV with... 

Nachon, R, Carletti, E., Wandhammer, M., et al., 2011. X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger. J. Biochem. 434, 73. 

Geyer, B.C., Kannan, L., Cherni, I., et al., 2010. Transgenic plants as a source for the bioscavenging enzyme, human butyrylcholinesterase. Plant Biotechnol. 

Ilyushin, D.G., Smirnov, I.V., Belogurov Jr., A.A., et al., 2013. Chemical polysi-alylation of human recombinant butyrylcholinesterase delivers a long-acting bioscavenger for nerve agents in vivo. Proc. Natl. Acad. Sci. USA 110,1243-1248. 

Cerasoli, D.M., Griffiths, E.M., Doctor, B.P., et al., 2005. In vitro and in vivo characterization of recombinant human butyrylcholinesterase (Protexia) as a potential nerve agent bioscavenger. Chem. Biol. Interact. 157, 363-365. 

Cohen, O., Kronman, C., Raveh, L., et al., 2006. Comparison of polyethylene glycol-conjugated recombinant human acetylcholinesterase and serum human butyrylcholinesterase as bioscavengers of organophosphate compounds. 

Rosenberg, Y.J., Saxena, A., Sun, W., et ah, 2010. Demonstration of in vivo stability and lack of immunogenicity of a polyethyleneglycol-conjugated recombinant CHO-derived butyrylcholinesterase bioscavenger using a homologous macaque model. Chem. Biol. Interact. 187, 279—286. 

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