Bioreactors, examples

Using the MiniTab output for regressicm of the data from Example 4.2 (Table 6.1 A), the bioreactor example, the origmal fuU model was... 

Methane has also been used in aerobic bioreactors that are part of a pump-and-treat operation, and toluene and phenol have also been used as co-substrates at the pilot scale (29). Anaerobic reactors have also been developed for treating trichloroethylene. Eor example, Wu and co-workers (30) have developed a successful upflow anaerobic methanogenic bioreactor that converts trichloroethylene and several other halogenated compounds to ethylene. 

Water Groundwater can be treated in anaerobic bioreactors that encourage the growth of sulfate reducing bacteria, where the metals are reduced to insoluble sulfides, and concentrated in the sludge. For example, such a system is in use to decontaminate a zinc smelter site in the Netherlands (95). 

Eig. 5. Examples of air driven bioreactors (a) bubble column, (b) draught tube, and (c) external loop. 

Loop reactors are particularly suitable as bioreactors to produce, for example, single-cell protein (96). In this process, single yeast or bacteria ceUs feeding on methanol multiply in aqueous culture broths to form high value biomass at 35—40°C, 20 kg/m ceU concentrations, and specific growth rates of... 

Where yield coefficients are constant for a particular cell cultivation system, knowledge of how one variable changes can be used to determine changes in the other. Such stoichiometric relationships can be useful in monitoring fermentations. For example, some product concentrations, such as CO2 leaving an aerobic bioreactor, are often the most convenient to measure in practice and give information on substrate consumption rates, biomass formation rates and product formation rates. 

Example 4 Oxygen Requirements for Activated Sludge in an Aerated Bioreactor... 

Surface interactions play an important role in the ability of certain animal cells to grow and produce the desired bioproducts. An understanding of the dynamics of cell surface interactions in these "anchorage-dependent" cells (cells that function well only when attached to a surface) will be needed, for example, to improve the design of bioreactors for growing animal cells. 

Another example was done by Opitz et al. They utilized P4HB scaffolds to produce viable ovine blood vessels, and then implanted the blood vessels in the systemic circulation of sheep. Enzymatically derived vascular smooth muscle cells (vSMC) were seeded on the scaffolds both under pulsatile flow and static conditions. Mechanical properties of bioreactor-cultured blood vessels which were obtained from tissue engineering approached those of native aorta. 

Natural pigment production for food coloration includes the entire spectrum of biotechnologies. For example, biological production of carotenoid pigments has medical implications because carotenoids are nutritive (pro-vitamin A), antioxidant, and photoprotective. Carotenoids are produced alternately in agricultural systems (plants), industrial bioreactors (bacterial and fungi), and marine systems (cyanobacteria and algae). 

As a second example let us consider the fed-batch bioreactor used by Ka-logerakis and Luus (1984) to illustrate sequential experimental design methods for dynamic systems. The governing differential equations are (Lim et al., 1977) ... 

A number of examples from biochemical engineering are presented in this chapter. The mathematical models are either algebraic or differential and they cover a wide area of topics. These models are often employed in biochemical engineering for the development of bioreactor models for the production of bio-pharmaceuticals or in the environmental engineering field. In this chapter we have also included an example dealing with the determination of the average specific production rate from batch and continuous runs. 

For each quantity that we want to maintain at some value, we need to ensure that the bioreactor is operating at the desired conditions. Let s use the pH as an example. In control calculations, we commonly use a block diagram to represent the problem (Fig. 1.2). We will learn how to use mathematics to describe each of the blocks. For now, the focus is on some common terminology. 

When we change a specific operating condition, meaning the set point, we would like, for example, the pH of the bioreactor to follow our command. This is what we call servo control. 

Examples for Metabolite Synthesis with a Microbial Bioreactor 200... 

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