Bioprocesses flow injection analysis

M. Reinecke and T. Scheper, Fast online flow injection analysis system for IgG monitoring in bioprocesses, J. Biotechnol., 59 (1997) 145-153. 

Spielman A, Garn M B, Aellen T, Haemmerlil S and Widmer H M 1992 Flow injection analysis methods for on-line bioprocess monitoring the automated measurement of some basic medium components and an enzymic product Microbial Principles in Bioprocesses Cell Culture Technology, Downstream Processing and Recovery (DECHEMA Biotechnology Conf. 5, Pt A) pp 367-70... 

Flow-injection analysis (FIA) has gained tremendous importance for bioprocess control during the last few years [23-27], Although this is not an in situ or a real on-line analytical technique, it can be automated and operated at very high analysis cycle frequencies (quasi online). One of the most important advantages of FIA is the use of very small sample volumes. 

Hitzmann, B., Gomersall, R., Brandt, J., van Putten, A. (1995) An expert system for the supervision of a multi channel flow injection analysis system. In Recent Advances in Biosensors, Bioprocess Monitoring, and Bioprocess Control, K.R. Rogers, A. Mulchandani, W. Zhou, Eds., ACS Symposium Series, American Chemical Society, Washington, D.C. (this vol.)... 

In the field of chemical analysis, biosensors have undergone rapid development over the last few years. This is due to the combination of new bioreceptors with the ever-growing number of transducers [1]. The characteristics of these biosensors have been improved, and their increased reliability has yielded new applications. Recently, a new technique of enzyme immobilization has been developed to obtain biosensors for the determination of enzyme substrates [2]. It is based on the enzyme adsorption followed by a crosslinking procedure. Therefore, a penicillin biosensor can be obtained and associated with a flow injection analysis (FIA) system for the on-line monitoring of penicillin during its production by fermentation [3-4]. This real-time monitoring of bioprocess would lead to optimization of the procedure, the yield of which could then be increased and the material cost decreased. 

Chapters 9-16 cover the application of optical and biosensor methods for on-line monitoring and control of bioprocesses. Topics include the use of expert systems for controlling flow injection analysis systems and hybrid process modeling for bioprocess control. 

See also Blood and Plasma. Clinical Analysis Glucose. Enzymes Immobilized Enzymes Enzyme-Based Electrodes Enzymes in Physiological Samples Industrial Products and Processes. Ethanol. Flow Injection Analysis Principles Detection Techniques. Food and Nutritional Analysis Alcoholic Beverages. Forensic Sciences Alcohol in Body Fluids. Immunoassays, Techniques Enzyme Immunoassays. Lipids Determination in Biological Fluids. Pesticides. Process Analysis Bioprocess Analysis. 

S Haemmerli, A Schaeffler, A Manz and H M Widmer, An Improved Micro Enzyme Sensor for Bioprocess Momtonng by Flow Injection Analysis, Sensors and Actuators B, 1 (1992), 404-407... 

Schmidt HL (1993) Biosensors and flow injection analysis in bioprocess control. J Biotechnol... 

---