Cells bioprocessing

Several of the problems associated with whole cell bioprocesses are related to the highly effective metabolic control of microbial cells. Because cells are so well regulated, substrate or product inhibition often limits the concentration of desired product that can be achieved. This problem is often difficult to solve because of a poor understanding of the kinetic characteristics of the metabolic pathway leading to the desired product. 

In whole cell bioprocesses, extracellular products are preferable because this removes the requirement for cell disruption and tins reduces the level of impurities in the product solution. Nevertheless, product isolation and purification can be prohibitively expensive particularly for low concentration product streams, which is a feature of many bioprocesses. 

Kalogerakis, N. and L.A. Behie, "Oxygenation Capabilities of New Basket-Type Bioreactors for Microcarrier Cultures of Anchorage Dependent Cells", Bioprocess Eng., 17, 151-156(1997). 

Domenburg H. (2009) Progress in kalata peptide production via plant cell bioprocessing. Biotechnol J 4 632-645. 

A computer-aided on-line monitoring system was established and applied for plant cell bioprocesses, with Perillafrutescens as a model cell [86]. The cell concentration was successfully measured on-fine and in situ with a laser turbidi-... 

Tramper, J, Smit D, Straatman J Vlak JM (1988) Bioreactor bubble-column design for growth of fragile insect cells. Bioprocess Engineering 3 37-41. 

Keywords. In situ product removal (ISPR), Integrated bioprocessing. Whole cell bioprocesses... 

A significant number of general reviews on ISPR techniques in whole cell bioprocessing have been published [1,3,14-21]. Furthermore,more specialized reviews exist that cover either a certain product category such as ethanol [4] or butanol [22,23] or the use of certain specialized ISPR techniques such as aqueous two-phase system [24,25], organic-aqueous two-phase systems [26-30],or solid adsorbents [31]. 

Crowley J. (2(X)4) Using Sound waves for cGMP manufacturing of a fusion protein with mammalian cells. Bioprocess. Int., 2(3) 46-50. 

Fernandes TG, Rodrigues CAV, Diogo MM, Cabral JMS. (2014) Stem cell bioprocessing for regenerative medicine. J. Chem. Technol. BiotechnoL, 89 34-47. doi 10.1002/jctb.4189. 

BIO Mechanical disruption of cells Intracellular substances such as Baker s yeast cells Bioprocessing [25]... 

Production of Alkaloids Via Plant Cell Bioprocessing Pregenomic Approach. 224... 

Fong WJ, Tan HL, Choo A, Oh SK. 2005. Perfusion cultures of human embryonic stem cells. Bioprocess Biosyst 27(6) 381-7. 

Cotter, J.L., Chinn, M.S., Grtmderr, A.M., 2009a. Ethanol and acetate production by Clostridium ljungdahlii and Clostridium autoethanogenum using resting cells. Bioprocess and Biosystems Engineering 32 (3), 369—380. 

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