Bioluminescence techniques

Chemiluminescence and Bioluminescence Techniques Fluorescence Spectroscopy to Study Molecular Recognition Proteins in Cellular Systems, Chemical Tools to Study Spectroscopic Techniques Proteins... 

Chemical Labeling Overview of Applications in Chemical Biology Chemiluminescence and Bioluminescence Techniques Fluorescence in Living Systems Overview of Applications in Chemical Biology... 

Voevodina T, Kovalevskii A, Kratasyuk V, Schultz V, Nifantyev O. Bioluminescent technique to analyse degree of endotoxication. In Jezowska-Trzebiatowska B, Kochel B, Slawinski J, Strek W, eds. Biological luminescence. World Scientific, Singapore 1990 573- 81. 

Theron DP, Prior BA, Lategan PM. Sensitivity and precision of bioluminescent techniques for enumeration of bacteria in skim milk. J Food Prot 1986 49 8-11. 

LJ Kricka. Chemiluminescent and bioluminescent techniques. Clin Chem 37 1472, 1991. 

The goal of our investigation was to apply available bioluminescent techniques for investigation of the early stages of interaction of bacterial pathogens with the host cell... 

K26. Kricka, L. J., Chemiluminescence and bioluminescence techniques. Clin. Chem. (Winston-Salem, N.C.) 37, 1472-1481 (1991). 

Because of rapid response time, luciferin-luciferase bioluminescence techniques are increasingly being used in lieu of traditional swab and plate methods. These require specialized monitoring equipment as well as sample collection and preparation supplies and chemicals (see Appendix C.4.6). 

The bioluminescence technique is applied for quantitative measurement of tumor burden, treatment response, immune cell trafficking, and detection of gene transfer [40]. The luminescence property depends on adenosine triphosphate and oxygen-dependent enzymatic conversion of exogenous luciferin to oxyluciferin by luciferase within living cells. An emission spectrum is produced with a peak of approximately 560 nm, which can be detected by a highly sensitive charge coupled device camera at 10-15 min after injection of luciferin and lasts for 60 min in mice [41]. 

However, the linear bond cleavage hypothesis of the firefly bioluminescence was made invalid in 1977. It was clearly shown by Shimomura et al. (1977) that one O atom of the CO2 produced is derived from molecular oxygen, not from the solvent water, using the same 180-labeling technique as used by DeLuca and Dempsey. The result was verified by Wannlund et al. (1978). Thus it was confirmed that the firefly bioluminescence reaction involves the dioxetanone pathway. Incidentally, there is currently no known bioluminescence system that involves a splitting of CO2 by the linear bond cleavage mechanism. 

Girsch, S. J., and Hastings, J. W. (1978). The properties of mnemiopsin, a bioluminescent and light sensitive protein purified by hollow fiber techniques. Mol. Cell. Biochem. 19 113-124. 

There are cAMP assays that can be measured using a variety of techniques [35], including HTRF (CisBio) [36], bioluminescence (cAMP-Glo, Promega) [37], TR-FRET (CisBio) [38], LANCE [39], Alphascreen (Perkin Elmer) [40] and Enzyme Fragment Complementation (EFC) [41] (Hithunter, DiscoverX). This review will focus on the LANCE, the TR-FRET and ECF assays as they are the most used in our laboratories. 

Synthetic luciferin as well as purified preparations of native and recombinant firefly luciferases are now commercially available allowing the bioluminescent determination of ATP to be used as a routine analysis technique in some laboratories. 

Nicolas, J.-C., Balaguer, R, Terouanne, B., Villebrun, M.A., and Boussioux, A.-M. (1992) Detection of glucose 6-phosphate dehydrogenase by bioluminescence. In Nonisotopic DNA Probe Techniques (L.J. Kricka, ed.), p. 207. Academic Press, New York. 

With the newly proposed detector, Dadoo et al. [84] adapted this bioluminescence reaction to determine ATP. A selective and sensitive determination is achieved because the use of CE as a separation technique minimizes the effect of several interfering substances such as some anions (e.g., SCN, I ) that inhibit the reaction decreasing the luminescence emission, and even some nucleotides that generate light in this reaction but with lower intensity. A detection limit of 5 nM, approximately 3 orders of magnitude lower than using UV detection, was obtained. 

For more than 30 years, the phenomenon of luminescence—originally a curiosity in the physical laboratory—has been the basis of a well-established and widely applied spectrometric branch of analytical chemistry. Specifically, chemiluminescence (CL)-based analysis is growing rapidly, offering a simple, low-cost, and sensitive means of measuring a variety of compounds. Owing to elegant new instrumentation and, especially, to new techniques, some of which are entirely new and some borrowed from other disciplines, CL and bioluminescence (BL) can now be routinely applied to solve diverse qualitative and quantitative analytical problems. 

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