Bethesda assay

The Bethesda assay is used to detect levels of inhibitory antibodies. One Bethesda unit is the reciprocal of the dilution of test plasma at which 50% of normal activity is inhibited. Briefly, the test plasma sample is mixed with an equal volume of pooled normal plasma and incubated at 37 °C for 2 h. Residual activity is measured by aPTT as described above (Herzog et al., 1999 Chao et al., 2001 Fields et al., 2001 Gallo-Penn et al., 2001). 

Yang LL. 1988. CHO/HGPRT mutation assay. Study No. T5196.332. Microbiological Associates Inc., Bethesda, MD. 

HCT-116 human colon carcinoma (ATCC, Bethesda MD) cells were grown in McCoy s 5A) and were routinely subcultured twice weekly. Antiproliferative assay was performed by chemoluminescence assay based on quantification of ATP. Cells in their exponential phase of growth were treated at different times (lh or 24h) with different concentrations of edotecarin or SN-38. For post-treatment recovery studies, cells were washed with PBS and left in drug-free culture medium. Then, cell medium was collected to avoid any cell loss. Cells in monolayer were washed, detached with trypsin, and collected in the medium. Cells were counted in a Multisizer 3 Coulter Counter to measure the drug s effects on growth inhibition. Samples were fixed either... 

The histamine- and leukotriene B4-releasing potential of the RCBPA P743 has been investigated in vitro on the RBL-2H3 mast cell line [25]. RBL-2H3 cells (with permission of Dr RP Siraganian, National Institutes of Health, Bethesda, MD, USA) were replated on Eagle s minimum essential medium and incubated for 30 minutes at 37 °C in 5% CO2 with the test-solutions. Calcium ionophore A23187 was used as a positive control. The viability of mast cells was determined by means of a quantitative colorimetric assay that is based on the abiUty of viable cells to cleave the reagent MTT [33]. 

Reid Y, Storts D, Riss T, Minor L (2004-2013). Authentication of hitman cell lines by STR DNA profiling analysis. In Sittampalam GS, Gal-Edd N, Arkin M et al (eds) Assay guidance manual [Internet], Eli Lilly Company and the National Center for Advancing Translational Sciences, Bethesda, MD. Available http //www.ncbi.nlm.nih.gov/ books/NBKl44066/... 

OVCAR is a human ovarian cell line that expresses STn on the cell surface. OVCAR was obtained from American Type Culture Collection (Bethesda, MD), grown and then placed in standard chromium release assay 17). Mononuclear cells from patients were incubated in the presence or absence of Interleukin 2 (IL2) (Genzyme, Cambridge, MA) at a concentration of 10 cells/ml with 100 units of IL2 for 48 hours -72 hours. Cells were washed and used as effector cells in a standard four hour killing assay as previously described (77). Fifty three... 

Much less expensive point detectors are available as prototype "One Step Hand-Held Assay" devices. These instruments are currently produced by the Navy Medical Research Institute (NMRI) at Bethesda, Maryland,... 

Haas J et al (2004) In vivo assay guidelines. In Sittampalam GS et al (eds) Assay guidance manual. Eli Lilly Company, Bethesda, MD... 

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