Assay guidance manual

SMILES simplified molecular input line entry system http //www.daylight.com/smiles/index.html. National Institutes of Health Assay Guidance Manual Version 5.0 http //www.ncgc.nih.gov/guid-ance/manual toc.html. 

Additionaf reading on the subject of assay validation and specific protocols can be found online at the NCGC web site Assay Guidance Manual (http //www.ncgc.nih.gov/guidance/index.html). 

Reid Y, Storts D, Riss T, Minor L (2004-2013). Authentication of hitman cell lines by STR DNA profiling analysis. In Sittampalam GS, Gal-Edd N, Arkin M et al (eds) Assay guidance manual [Internet], Eli Lilly Company and the National Center for Advancing Translational Sciences, Bethesda, MD. Available http //www.ncbi.nlm.nih.gov/ books/NBKl44066/... 

Assay Guidance Manual, version 4.1. Available www.ncgc.nih.gov/guidance. 

In this section, we outline what considerations have to be taken when planning to screen for compounds that induce phenotypic changes of interest in C. elegans. While the details are highly dependent on the size of the screen, the phenotype of interest, and the assay conditions, there are some general considerations that are common to all screens. In addition to the recommendations that are provided below, a good place to find information on how to develop an in vivo screen is the Assay Guidance Manual that can be found in the NIH bookshelf [I]. 

Haas J et al (2004) In vivo assay guidelines. In Sittampalam GS et al (eds) Assay guidance manual. Eli Lilly Company, Bethesda, MD... 

Creatinine method check. At least once daily run a CREA test on a solution of known creatinine activity such as an assayed control or calibration standard other than that used to calibrate the CREA method. For further details review the Quality Assurance Section of the Chemistry Manual. The result obtained should fall within acceptable limits defined by the day-to-day variability of the system as measured in the user s laboratory. (See SPECIFIC PERFORMANCE CHARACTERISTICS for guidance.) If the result falls outside the laboratory s acceptable limits, follow the procedure outlined in the Chemistry Troubleshooting Section of the Chemistry Manual. 

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