Hand held assays

One of the first bioagent detection technologies fielded in the early 90 s was the immunochromatographic assay (also known as Hand Held Assays, Figure 2). The immunochromatographic assays include a nitrocellulose strip to which a liquid sample is added at one end. As the liquid flows down the... 

Much less expensive point detectors are available as prototype "One Step Hand-Held Assay" devices. These instruments are currently produced by the Navy Medical Research Institute (NMRI) at Bethesda, Maryland,... 

Figure 1. Typical hand held assay test (4). (see page 1 of color inserts)...

Figure 2. Schematic of positive and negative hand held assay tests. A. Target is present in the applied sample. B. The presence of target results in the formation of two purple lines on the test. C. No target is present in the applied sample. D. The lack of target results in the formation of one purple line on the test (4). (see page 1 of color inserts)...

The combination of bacterial display libraries with CTMACS allows the rapid production of affinity reagents, see Figure 5. These, in turn, are used in the production of immunochromatographic hand held assays. The result is high throughput production and deployment of simple to use assays for the most relevant biological and chemical warfare agents. 

A target sample solution and a negative control are pipetted onto separate hand held assays near the gold nanoparticles. The sample with target should produce two purple hues and the negative control should produce one purple line. Any non-bound nanoparticles will continue to flow to the end of the... 

Figure 10. Hand held assay test for C-Reactive Protein. The leftmost line is the positive control and the rightmost line is the sample line (4).

Figure 11. Cell based hand held assay for the detection ofT7 antibodies.

Figure 1. Typical hand held assay test (4).

E691 Wilding, P. and Erickson, K.A. (1990). Evaluation of a hand-held micro-fabricated sensor system, the i-STAT 100, for rapid assay of electrolytes, glucose, urea nitrogen and hematocrit. Clin. Chem. 36, 1206, Abstr. 1185. 

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