Bacteria enrichment media

Luminous bacteria are bioluminescent microorganisms whose luciferase genes (lux), proteins and intact cells are widely used in applied research and commercial products. Acknowledging the commercial value of luminescent cells also in entertainment and education, we have conducted research on luminous bacteria from marine samples and have isolated Photobacterium phosphoreum (strain RL-1) from coastal marine sediment. In order to maximize the luminescence activity of RL-1, we examined a series of extracts prepared from dried marine foodstuff. Because chitinous compounds and some amino acids are known to be abundant in dried squid and shrimp, we also tested the effects of those compounds on the luminescence activity. Among the supplemental compounds tested, chitosan, cysteine, and aspartic acid were found to enhance the luminescence activity of RL-1. The present results indicate that some amino acids and chitinous compounds are effective supplements for further enhancing bacterial light production in an enriched medium (SWC ). 

Function. An obvious function of methane monooxygenase is the oxidation of methane to methanol. The membrane-bound form could fulfill this function within an electron-transport chain. A further, possible function could be derived from the observation that, in methanotrophic bacteria grown in copper-enriched medium, the main mass of membrane-bound proteins is methane... 

Enzyme from Mn-deficient cells showed no ribonucleotide reductase activity but could be activated by addition of Mn (215). Furthermore, Mn was incorporated into the B2 subunit when the bacteria were grown on MnCL-enriched medium (8). These experiments strongly implicate a manganese containing active site. 

Differences in growth phase can influence metal uptake in batch-cultured bacteria. Germanium uptake by Pseudomonas putida occurs in a biphasic pattern in a catechol-enriched medium the second uptake phase corresponds to catechol degradation, products of which facilitate germanium transport into cells [35]. 

Two bacterial strains, one from soil and the other from infected local beer, which utilised calarene as the sole source of carbon and energy have been isolated by enrichment culture techniques [149]. Both these bacteria were adapted to grow on valencene as the sole carbon source. Fermentations of valencene (5) by these bacteria of the genus Enterobacter in a mineral salts medium yielded several neutral metabolic products dihydro alpha-agarofuran (200) (7.5%), nootkatone (6) (12%), another ketone (201) (18%) and a-cyperone (202) (8%), Fig. (40). 

FIGURE 2 Degradation of pullulan (MW 200,000) in replicate anaerobic enrichment cultures of marine bacteria from anoxic sediments. Open circles show total pullulan concentrations remaining in the medium at each time point. The molecular weight distribution of the pullulan is shown by the stacked bars white >10,000 Da, stripes 5000 Da, black <1200 Da. Note that the lower molecular weight fraction progressively accumulated between 50 and 64 h. [Data from Arnosti et al. (1994).]... 

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