Bacillus pumilus

Jandyala D, M Berman, PD Myers, BT Sewell, RC Willson, MJ Benedik (2003) CynD, the cyanide dihydratase from Bacillus pumilus gene cloning and structural studies. Appl Environ Microbiol 69 4794-4805. 

Dickinson, D. N. La Due, M. T. Satomi, M. Winefordner, J. D. Powell, D. H. Venkateswaran, K. MALDI-TOF MS compared with other polyphasic taxonomy approaches for the identification and classification of Bacillus pumilus spores. J. Microbiol. Meth. 2004, 58,1-12. 

Wang, W. Liu, Z. Ma, L. Hao, C. Liu, S. Voinov, V. G. Kalinosvskaya, N. I. Electrospray ionization multiple-stage tandem mass spectrometric analysis of digycosyldiacylglycerol glycolipids from the bacteria Bacillus pumilus. Rapid Comm. Mass Spectrom. 1999,13,1189-1196. 

Meyers PR, Rawlings DE, Woods DR, et al. 1993. Isolation and characterization of a cyanide dihydratase from Bacillus pumilus Cl. J Bacteriol 175(19) 6105-6112. 

From the fore gut of Laccophilus minutus, a Bacillus pumilus strain was isolated which produced maculosin, the diketopiperazine formed from proline and tyrosine [103] 24, phenyl malonate 25, N-acetylphenylalanine 26, N-acetyl-tryptophane 27 and 3,4-dihydroxybenzoic acid [ 103]. Maculosin which has also been isolated from several microorganisms and sponges shows phytotoxic and cytotoxic properties [103], 3,4-dihydroxybenzoic acid shows antioxidant properties and was already found in pygidial defensive glands of several dytis-cid beetles. 

Figure 1. Amino acid sequences of microbial glycohydrolases. A Aureobasid-iwn sp. endo-xylanase Sc Schizophyllwn commune endo-xylanase C Chainia sp. endo-xylanase Bp Bacillus pumilus endo-xylanase Bs Bacillus subtilis Bacillus circulans endo-xylanase Pf Pseudomonas fluorescens endo-xylanase B alkalophilic Bacillus sp. endo-xylanase Ct Clostridium thermocellum endo-xylanase Cf Cellulomonas fimi cellobiohydrolase Ca Cryptococcus albidus endo-xylanase. Residue numbers are those of the adjacent residue, counting from the N-terminus of the mature protein.

Kalinovskaya. N.I. Kuznetsova, T.A. Rashkes, Ya.V. Mil grom, Yu.M. Mil grom, E.G. et al. (1995) Surfactin-like structures of five cyclic depsipeptides from the marine isolate of Bacillus pumilus. Russ. Chem. Bull., 44, 951-5. 

Later studies concerned the detection of both sulfonamides and sulfonamide potentiators in fish tissues. Mixtures containing ethyl acetate, sodium sulfate, and sodium hydroxide (80), or ethyl acetate and sodium hydroxide (115), have been all used for extracting residues of sulfadiazine and trimethoprim from trout and salmon tissues. In the former approach, para-aminobenzoic acid was employed to neutralize sulfadiazine prior to the microbiological assay of trimethoprim, whereas in the latter approach Bacillus subtilis (ATCC) was directly used for sulfadiazine detection. The limit of detection for sulfadiazine using Bacillus subtilis was found to be 0.04 ppm. The test indicator organism used in both approaches to detect trimethoprim down to 0.1 ppm was Bacillus pumilus (CN60 Welcome Research Laboratories, London) (115,116). 

T. Kasumi, Y. Tsumuraya, C. F. Brewer, H. Kersters-HiIderson, M. Claeyssens, and E. S. Hehre, Catalytic versatility of Bacillus pumilus 3-xylosidase Glycosyl transfer and hydrolysis promoted with a- and fJ-D-xylosyl fluoride, Biochemistry 26 3010 (1987). 

The micro-organism used for TC is Bacillus pumilus NCTC 8241 and Bacillus cereus ATCC 11778. Buffer solutions of pH 4.5 in both cases and the incubation temperatures of 37 - 39° and 35 - 37 °C were used respectively (90). 

Degrassi, G., Vindigni, A., and Venturi, V, A thermostable a-arabinofuranosidase from xylanolytic Bacillus pumilus purification and characterisation. J Biotechnol 2003, 101 (1), 69-79. 

Bacillus licheniformis NCTC 10341 Bacillus polymyxa NCTC 4747 Bacillus pumilus NCTC 8241 Bacillus subtilis ATCC 6633, MP 1 Bacillus subtilis UC 564 Bacillus sphaericus NCTC 431... 

On plates having Bacillus pumilus 8241, Cf (5 xg) and Fp (200 xg) discs when placed separately and independently, the zones of inhibition were 23.1 mm and 18.0 mm, respectively, which increased to 25.9 mm with Cf and 22.0 mm with Fp, when the discs were placed for the elicitation of combined effect. The percent increase in surface area towards Cf was found to be 25.71%, while for Fp it was 49.38%, thereby enhancing the activity between the drugs and establishing a state of synergism (Table 40). 

Bacillus pumilus strains Cl and Cyanide dihydratase Jandhyala et al. (2003)... 

Cyanide dihydratases are also HCN specific, but differ in the reaction mechanism, which leads to formate. Their phylogenetic distribution is also different these enzymes occur in bacteria, for instance. Bacillus pumilus [21] or Pseudomonas... 

A one-pot efficient method for the synthesis of derivatives 67 and 68 by condensation reaction of barbituric acids, lH-pyrazol-5-amines and aldehydes under solvent-free conditions has been reported (Scheme 16) [39]. These products were evaluated in vitro for their antibacterial activities against E. coli (ATCC 25922), P. aeruginosa (ATCC 85327), Enterococcus faecalis (ATCC 29737), Bacillus subtilis (ATCC 465), Bacillus pumilus (PTCC 1114), Micrococcus luteus... 

The rRNA catalogs for the various eubacterial phyla do not permit us to infer a branching order, because the 5 8 (association coefficient) values between the major branches are too low to be significant. For example, between members of the green sulfur bacteria and members of the filamentous photosynthetic bacteria 5 8 values range from 0.12 to 0.20 [111]. These values are too low to establish branching orders with respect to other phyla , for example Escherichia coli (purple bacteria and relatives, 5 8 = 0.17-0.28) and Bacillus pumilus (gram-positive bacteria, 5 8 = 0.18-0.24). As shown in Fig. 1, the various phyla disappear into... 

Pumiacidins A, B, C, D, E, F and G (Fig. 5) were isolated by Naruse et al. in 1990 from Bacillus pumilus. Pumilacidins show no inhibitory activity against bacteria and fungi at l,000ng/ml by paper-disk assay. These compormds inhibit herpes virus typel and K -ATPase, and demonstrate anti-ulcer activity in rats ... 

Hamada, Y., Hara, O., Kawai, A., Kohno, Y., Shioiri, T. New methods and reagents in organic synthesis. 97. Efficient total synthesis of Al-77-B, a gastroprotective substance from Bacillus pumilus AI-77. Tetrahedron 99, 47, 8635-8652. 

Letelyan azide-free synthesis of oseltamivir phosphate was reported, where the key intermediate is aminoshikimic add produced by a two-step microbial process Bacillus pumilus to generate kanosamine followed by engineered E. coli to give aminoshikimic acid (Scheme 8.20) [81-83]. 

---