B-cell line

PCI-32765 1 is the only Btk inhibitor which has been reported to have advanced to clinical trials [40]. Modeling of pyrazolopyrimidine 2 suggested that replacement of the cyclopentyl moiety could position an electrophilic group in proximity to Cys481, and subsequent optimization led to 1 [41]. Compound 1 inhibits Btk with an IC50 of 0.8 nM, and covalent binding to Btk was confirmed by mass spectrometry and washout experiments. In the Ramos B-cell line, 1 inhibits BCR-induced calcium... 

Filippini, G. et al., Flow cytometric detection of p53 protein after incubation of a pre-B cell line with antitumor agents, Cytometry, 35, 267, 1999. 

Mature B lymphocytes express AhR and ARNT,10,12 and the ability of TCDD to suppress primary antibody responses has been known for a long time (reviewed in4). While the precise mechanisms resulting in suppression of T-dependent B cell responses in vivo have not been fully elucidated, it is clear that direct effects of TCDD on B cells plays a role. Studies using two B cell lines with differential AhR expression have linked suppression of LPS-induced IgM production to AhR activation, and have shown that AhR interactions within the 3 alpha immunoglobulin heavy chain enhancer directly influence antibody production.52-54 TCDD also appears to directly modulate the expression of CD19 on human B cells, suggesting another mechanism by which TCDD down-modulates the responsiveness of mature B cells.55... 

Thrasher, A., Chetty, M., Casimir, C., Segal, A. W. (1992). Restoration of superoxide generation to a chronic granulomatous disease-derived B-cell line by retrovirus mediated gene transfer. Blood 80,1125-9. 

Hartmann U, Balabanov S, Ziegler P et al. Telomere length and telomerase activity in the BCR ABL transformed miuine Pro B cell line BaF3 is unaffected by treatment with imatinib. Exp.Hematol. 2005 33 542 549. 

Fruci, D., Ferracuti, S., Limongi, M. Z., Consolo, V., Giorda, E., Fra ioli, R., Sibilio, L., Carroll, 0., Hattori, A., van Endert P. M., and Giacomini, P., 2006, Expression of endoplasmic reticulum aminopeptidases in EBV B cell lines from healthy donors and in leucemia/lymphoma, carcinoma, and melanoma cell lines, J. of Immunol. 176 4869-4879. 

Takami, Y. and Nakayama, T. (1997) A single copy of linker HI genes is enough for proliferation of the DT40 chicken B cell line, and linker HI variants participate in regulation of gene expression. Genes Cells 2, 711-723. 

M. Sanchez Dominguez, M.P. Krafft, E. Maillard, S. Siegrist, A. Belcourt, Prevention of adhesion and promotion of pseudoislets formation from a b-cell line by fluorocarbon emulsions, ChemBioChem 7 (2006) 1160-1163. 

Subramaniam, P.S., EE. Cruz, A.C. Hobeika, and H.M. Johnson, Type I interferon induction of the Cdk-inhibitor p21WAFl is accompanied by ordered G1 arrest, differentiation and apoptosis of the Daudi B-cell line. Oncogene, 1998.16(14) 1885-90. 

By fusing an immortal mouse myeloma B-cell line and an immune B-lymphoblast containing a gene that expressed a specific... 

Hacein-Bey, H., M. Cavazzana-Calvo, F. Le Deist, A. Dautry-Varsat, C. Hivroz, I. Riviere, O. Danes, J.M. Heard, K. Sugamura, A. Fischer, and G. De Saint Basile, gamma-c gene transfer into SCID XI patients B-cell lines restores normal high-affinity interleukin-2 receptor expression and function. Blood, 1996. 87(8) 3108-16. 

Not all data favored stepwise linear deletion as the basis for switching. Obata et al. (1981) found that the DNA organization in a particular plasmacytoma could be explained by unequal sister chromatid exchange, but observations inconsistent with this possibility were reported by Wabl et al. (1985). Alt et al. (1982) observed that a subclone of a transformed pre-B cell line had switched from synthesis of a p chain to synthesis of a y chain, with retention of the same VH and JH as well as both copies of the gene these results suggested that the switch in heavy chain synthesis was a transcriptionally controlled event (see discussion below). 

Reth, M.G., Ammirati, P., Jackson, S., Alt, F.W. (1985). Regulated progression of a cultured pre-B-cell line to the B-cell stage. Nature 317,353-355. 

Shapiro, A.M., Schlissel, M.S., Baltimore, D., De Franco, A.L. (1993). Stimulation of k light-chain gene rearrangement by the immunoglobulin p heavy chain in a pre-B-cell line. Mol. Cell. Biol. 13, 5679-5690. 

Williams, P.B., Kubo, R.T., Grey, H.M. (1978). p-Chains from a nonsecretor B cell line differ from secreted p-chains at the C-terminal end. J. Immunol. 121, 2435-2439. 

Yancopoulos, G.D., Desiderio, S.V., Paskind, M., Reamey, J.F., Baltimore, D., Alt, F.W. (1984). Preferential utilization of the most / -proximal VH gene segments inpre-B-cell lines. Nature 311, 727-733. 

Haan, K.M., Aiyar, A. and Longnecker, R. (2001) Establishment of latent Epstein-Barr virus infection and stable episomal maintenance in murine B-cell lines. J. Virol., 75, 3016-3020. 

Recently, Sedqi et al. [24] were able to clone a delta opioid receptor complementary DNA by expression of cDNA library from activated thymocytes in Cos 7 cells, whose amino acid sequence was similar to the neural counterpart. Interestingly, they also observed that transcripts for kappa and mu opioid receptors were not detected in thymocytes. Furthermore, Gave-riaux et al. [25] demonstrated transcripts for the delta opioid receptor in T-lymphocyte, B-lymphocyte, and monocyte cell lines, as well as in murine splenocytes. However, they observed that the kappa opioid receptor transcript was only found in B-cell lines. These studies may suggest a selective expression of the delta opioid receptor in specific cells and tissues of the immune system and suggests specialized functions in different anatomical regions. 

Maly, F.-E. Nakamura, M. Gauchat, J.-F. Urwyler, A. Walker, C. Dahinden, C.A. Cross, A.R. Jones, O.T.G. Week, A.L. Superoxide-dependent nitroblue tetrazolium reduction and expression of cytochrome 6245 components by human tonsillar lymphocytes and B cell lines. J. Immunol. 1989, 142, 1260-1267. 

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