Atrazine acetic acid

Milk Isotachophoresis Conductivity 2ng 40% methanol, 10 mM sodium acetate pH 4.8, 0.2% hydroxy-ceUulose (leading electrolyte), 40% methanol, 20 mM acetic acid (terminating electrolyte) Prometryne, desmetryne, terbutryne, atrazine (OH metabolites), simazine (OH metabolites) 126... 

Kavetskii et al. [224] developed a method for the simultaneous determination of pesticides in soil. A combination of thin layer chromatography and gas chromatography was used. The pesticides examined were 4,4 DDT, 4,4 DDD, 4,4 DDE, 2,4 DDT, GHCG, aGHCG, Metaphos, Phosphamidon, Phozalone, Atrazine, Prometryne, Simazine and 2,4 dichlorophenoxy acetic acid. Detection limits were in the range 0.5-5pg kgy1. 

Acetamido-4-amino-6-chloro-s-triazine, see Atrazine Acetanilide, see Aniline, Chlorobenzene, Vinclozolin Acetic acid, see Acenaphthene, Acetaldehyde, Acetic anhydride. Acetone, Acetonitrile, Acrolein, Acrylonitrile, Aldicarb. Amyl acetate, sec-Amyl acetate, Bis(2-ethylhexyl) phthalate. Butyl acetate, sec-Butyl acetate, ferf-Butyl acetate, 2-Chlorophenol, Diazinon. 2,4-Dimethylphenol, 2,4-Dinitrophenol, 2,4-Dinitrotoluene, 1,4-Dioxane, 1,2-Diphenylhydrazine, Esfenvalerate. Ethyl acetate, Flucvthrinate. Formic acid, sec-Hexyl acetate. Isopropyl acetate, Isoamyl acetate. Isobutyl acetate, Methanol. Methyl acetate. 2-Methvl-2-butene. Methyl ferf-butvl ether. Methyl cellosolve acetate. 2-Methvlphenol. Methomvl. 4-Nitrophenol, Pentachlorophenol, Phenol. Propyl acetate. 1,1,1-Trichloroethane, Vinyl acetate. Vinyl chloride Acetoacetic acid, see Mevinphos Acetone, see Acrolein. Acrylonitrile. Atrazine. Butane. 

Chemicals processed. Waste pesticide solutions were collected after spray operations during late May, June, and July of 1983, and consisted primarily of three compounds 2,4-D [(2,4-dichloro-phenoxy)acetic acid], atrazine (2-chloro-4-(ethylamino)-6-(isopropylamino)- -triazine) and paraquat (1,1 -dimethyl-4,4 -bipyridinium dichloride). Our efforts were primarily directed at these pesticides, which are shown in Table I together with their formulations and concentrations. 

FIGURE 11.17 PCA loadings for raw mean-centered augmented data matrix from top to bottom, first to fifth (PCI to PC5) principal components. Compound names and abbreviations are as follows alachlor (ALA), atrazine (ATR), bentazone (BEN), biphenyl (BIF), 3-chlorophenol (3-CP), 4-chlorophenol (4-CP), (2,4-dichlorophenosy)acetic acid (2,4-D), dichloroprop (DCP), dimethoate (DIM), linuron (LIN), h-chloro-z-methyphenoxyacetic acid (MCPA), mecoprop (MEC), 4-chloro-3-methylphenol (MEP), metholachlor (MET), pen-tachlorophenol (PCP), simazine (SIM), (2,4,5-trichlorophenoxy)acetic acid (2,4,5-T), tribu-tylphosphate (TBP), 2,4,6-trichlorophenol (TCP). 

Soil extract (20 g). Extract with 40 mL of acetonitrile/water (80 20). Acidify extract with acetic acid (1/100 with DI water), add 25 mL of acid to 5 mL of extract for a total of 30 mL. Atrazine, propazine, and simazine. 

The first synthetic herbicide12 was 2,4-dichlorophenoxy-acetic acid (11.7). The one used in largest volume today is atrazine, which is typical of a group of triazine herbicides. Glyphosate (very low mammalian toxicity) is used extensively to kill vegetation nonselectively.13 Alachlor is a typ-... 

Branson B12 sonic bath). The extracts were decanted, centrifhged (10 min, 10,000 X g) to remove particulate material, and 0.01 volume of glacial acetic acid was added. These solutions were applied to SCX aromatic sulfonic acid SPE columns (Analytichem) containing 300 mg of resin. The columns were washed with 6 ml of 1 M I HP04, and atrazine was then eluted with 2 ml of acetonitrile 0.1 M i HP04 1 1. The eluates were diluted to 5 ml with PBS-Tween, and dilutions were analyzed hy EIA. 

The electrochemistry of triazines is well documented.Polak and Volke described a dpp procedure for the determination of symmetrical and asymmetrical triazines (desmetryne, methoprotryne, prometryne, aziprotryne, terbutryne, atrazine, cyanazine, simazine, terbuty-lazine, metribuzine) in water. The herbicides are extracted into chloroform from the sample at pH 8 to 9. The detection limit in 0.02 M H2SO4 or acetic acid buffered solution at pH 2.5 is given as 10 M. 

Water samples containing 3 ppm levels of analytes yielded detectable peaks. Simazine, atrazine, and propazine were separated and quantitated using a C]g column (thermospray MS) and a 50/50 methanol/water (1% acetic acid) mobile phase. Here, peaks of lOOng injected were detected [214]. In a separate study, particle beam MS detection was also used with a C g column and a 70/30 methanol/water (1.7mg/L phenoxyacetic acid and 1% acetic acid) mobile phase. A 50 ng injected detection limit was reported. An excellent 10-min separation was achieved with this method. 

Chloroform-acetone (9 1) for atrazine, hexane-glacial acetic acid-diethyl ether (72 30 18) for 2,4-D, and methanol-acetonitrile-tetrahydrofuran-water (50 15 8 27) for chlorpropham. 

Figure 3. PCR-DGGE of whole soil community 16S rDNA The soils were amended as follows WC, water control N NH4NO3 A, atrazine AN, atrazine + NH4NO3 CA, cyanuric acid ACA, atrazine + cyanuric acid ACN, atrazine + cyanuric acid + NH4NO3 Ace, acetate AA, atrazine + acetate AAN, atrazine + acetate + NH4NO3 P, pectin AP, atrazine + pectin APN, atrazine + pectin + NH4NO3 M91, Ralstonia hasilensis. Arrow indicates prominent band in atrazine-amended soils that did not receive ammonium nitrate.

Compound Name Atrazine Acetaldehyde Acetic Acid Ammonium Acetate n-Butyl Acetate Copper Acetate Dimethylacetamide Ethyl Acetate Isobutyl Acetate Isopropyl Acetate Methyl Acetate Nickel Acetate n-Propyl Acetate Sec-Butyl Acetate Zinc Acetate Acetaldehyde Acetic Anhydride Ethyl Acetate Ethyl Acetate Ethyl Acetoacetate Ethyl Acetoacetate Acetone... 

Isocyanatopropane, see Atrazine Isofenphos oxon, see Isofenphos Isoheptane, see PCB-1242 Isohexane, see PCB-1242 Isononane, see PCB-1242 Isononanes, see Octane Isooctane, see PCB-1242 Isooctanes, see Octane Isophthalic acid, see ro-Xylene Isopropoxyphenol, see ProDoxnr Isopropyl alcohol, see Isopropyl acetate Isopropylatnide, see Bentazone IV-Isopropylaniline, see Propachlor Isopropylbenzene, see Propylbenzene, PCB-1242,... 

Seven chlorotriazines (simazine, cyanazine, hydroxy- and deethyl-atrazine, atra-zine, deisopropylatrazine, chlorodiamino-.r-triazine) were baseline resolved in <14 min using a Cg column (thermospray MS) and a 20/80 —> 55/45 methanol/ water (50 mM ammonium acetate with 1% formic acid) gradient [216], Excellent peak shapes and good resolution were obtained. Detection limits in the 20 ng injected range were reported. The authors noted that these detection limits could not be achieved on a Cjg column. 

---