Applications molecular biotechnology

Microbes for Industiial and Agiicultural Applications, Dekker, NY, 1993. Click, B. R. and J. J. Pasternak, Molecular Biotechnology Trinciples and Applications of Recombinant DNA, ASM Press, Herndon, VA, 1994. Bajpai, Rakesb K., and Ales Prokop, eds. Recombinant DNA Technology II, Annals of the New York Academy of Sciences, vol. 721, 1993. 

Saha, B. C. (2000). Alpha-L-arabinofuranosidases Biochemistiy, molecular biology and application in biotechnology. Biotechnology Advances, Vol. 18, 5, (August 2000), pp. (403-423), ISSN 0734-9750... 

To understand the extraordinary potential for DNA to be utilized as a material in construction processes, the general properties of this biomolecule will first be discussed. In addition, examples of naturally occurring nucleic acid-based nanostructures will be described that are of great importance both for cellular processes and conventional applications in molecular biotechnology. 

Glick B.R Pasternak J.J. (1994) Molecular Biotechnology Principles and Applications of Recombinant DNA. Washington, D.C. American Society for Microbiology. 

Maulik, S. and Patel, S.D. (1997). Molecular Biotechnology Therapeutic Applications and Strategies. Wiley-Liss, New York. 

Click BR, Pasternak JJ, In Molecular Biotechnology Principles And Applications of Recombinant DNA (Eds Click BR, Pasternak JJ), pp. 145-169. American Society for Microbiology, Washington DC, 1998. 

An important feature of pharmaceutical research in the past few decades is that despite the substantial scientific achievements that have occurred, and that are reflected in the increasing application of biotechnology to new drug development, the rate of new product generation has been fairly steady (Scherer 2000 Cockburn 2004). As Cockburn acknowledges, however, counts of new molecular entities (NMEs) are a noisy measure. On average, over the long term, these numbers have been remarkably steady. 

Jaeger, K.E. and S.Wohlfarth. (1993) Bacterial lipases Biochemistry, molecular genetics and applications in biotechnology. Bioengineering, 9, 39-46. 

Prausnitz, J.M. (2003). Molecular thermodynamics for some applications in biotechnology. Journal of Chemical Thermodynamics, 35, 21-39. 

Glick BR, Pasternak JJ (1994) Molecular biotechnology principles and applications of recombinant DNA. ASM Press, Washington, DC... 

Collins, A.R. (2004). The comet assay for DNA damage and repair. Principles, applications and limitations. Molecular Biotechnology, 26,249-261. 

Due to the pendant carboxylic group, SAMs of lipoic acid have been used in a large number of surface modifications, providing access to new structure systems with potential applications in biotechnology and molecular electronics, etc. 

Click, B.R., and J. J. Pasternak. Molecular Biotechnology, Principles and Applications of recombinant DNA. 2nded. Waslungton American Society of Microbiology Press, 1998. 

An overview of the utilization of non-aqueous solvents in macromolecular applications has been presented. A variety of organic solvents have been used to characterize and understand the parameters necessary for their applications to biotechnology. Although no single parameter is predictive of a final utility, the critical parameters that continue to surface in these studies are the dielectric constant, hydrophobicity, dipole moment, viscosity and solubility factor of the solvent. Furthermore, the pH memory, molecular memory and water content of the solvent play important roles in stabilizing these molecules. Not all of... 

Muhammad Sohail and Edwin Southern describe in their contribution a different process for the creation of oligonucleotide arrays. Subsequently, they used the arrays in the screening for effective antisense reagents. This study is fascinating because of its impact on the understanding of interactions between nucleic acids, an interesting research area even after 50 years of structural DNA analysis, and in view of its practical implications for applications in biotechnology and molecular medicine. 

Functional Aptamers for Proteins and their Application in Biotechnology, Molecular Medicine and Diagnostics... 

In this review, we first briefly summarize pressure effects in molecular biology and applications in biotechnology. We then discuss some experimental techniques and finally we discuss in more detail results of studies on the high pressure structure and phase behavior of biomolecular systems, such as nucleic acids, lyotropic lipid mesophases, model biomembrane systems and proteins. 

It is known that many proteins and peptides aggregate into extended p sheet like structures [3], Formation of amyloid fibrils is recognized important not only in understanding of human diseases but also is found acceptable for development of ordered nanostructures with modifiable properties and applicable in biotechnology, material science, molecular electronics and related fields. 

Best, D.J. Applications of Biotechnology to Chemical Production. In Molecular Biology and Biotechnology. Walker, J.M., and Gingold, E.B. (eds) The Royal Society of Chemistry London, 1985, pp. 228-267. 

Applications in Biotechnology. Biopharmaceutical products must be well characterized by means of analytical techniques. RP-HPLC with UV detection is suitable for the content assay and impurity profiling because of its high resolution. However, the bioactivity of protein-based pharmaceuticals not only relies on the primary structure but also on the higher order structure, i.e., the molecular confirmation, which is not revealed by UV absorbance. Circular dichroism and intrinsic fluorescence detection have been combined with UV detection in RP-HPLC to monitor the conformational properties of active compounds and impurities in biopharmaceutical products [57]. 

PNAs are a class of DNA analogs with very promising properties for applications in molecular biotechnology or medicine. Compared to DNA, the ribose phosphate ester backbone is replaced by a pseudo-peptide backbone in... 

Deiman B, van Aarle E, and Sillekens P (2002) Characteristics and applications of nucleic acid sequence-based amplification (NASBA). Molecular Biotechnology 20 163-179. 

Lisby G (1999) Application of nucleic acid ampfification in clinical microbiology. Molecular Biotechnology 12 75-99. 

Figure 3 Ferguson plot of bovine v-crystallin proteins. Four different gel concentrations (5.0%, 6.25%, 7.3%, 8.3% T) with Tris-glycine gel buffer were used for determination of the slope, Kr, of each subtraction, which is proportional to the relative molecular mass, M of the native protein. (Reprinted with permission from Chiou SH and Wu SH (1999) Evaluation of commonly used electrophoretic methods for the analysis of proteins and peptides and their application to biotechnology. Analytica Chimica Acta 383 47-60 Elsevier.)...

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