Application methods immunocytochemistry

The first two design steps in the immunocytochemistry experiments are (1) whether to use fluorescence or enzyme-based labels and (2) which application method is... 

The application of immunocytochemistry to ammo acid transmitter systems m the central nervous system has provided valuable information on the cellular and subcellular locations of these transmitter systems It is particularly suited to the detection of the GABA system, and this topic forms the focal point of the chapter on immunocytochemical techniques. Although the im-munocytochemical approach is extremely valuable m specific situations, the method is of limited applicability m the amino acid field, only the GABA and taurine systems having been mapped by these procedures. 

De Mey J, Moeremans M. Raising and testing polyclonal antibodies for immunocytochemistry, in Immunocytochemistry, Modern Methods and Applications (Polack JM, Van Noorden S, eds.), Wright, Bristol, UK, 1986, pp. 3-12. 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

VanNoorden, S. (1986) Tissue preparation and immunostaining techniques for light microscopy, in Immunocytochemistry Modern Methods and Applications (Polak, J. M. and VanNoorden, S., eds.), Wright, Bristol, England, pp. 26-53. 

Bendayan, M. (1984) Protein A-gold electron microscopic immunocytochemistry methods, applications, and limitations. J. Electron Micros. Tech. 1, 243-270. 

Polak JM, van Noorden S, eds. Immunocytochemistry Modem Methods and Applications. 2nd ed. Bristol, UK Wright Publishing 1986. 

Uses or application - The methods where the antibody has been tried are generally any of the following immunohistochemistry (IHC), immunocytochemistry (ICC), or immunofluorescence (IF) western blot (WB) or immunoblot (IB) and immunoprecipitation (IP). This information should... 

Burry RW (1995) Pre-embedding immunocytochemistry with silver-enhanced small gold particles. In Hayat MA (ed.) Immunogold-sUver staining principles, methods and applications. Boca Raton, FL CRC Press, pp 217-230. 

This book, from its initial conception, had obviously to be limited in the choice of subjects, but we believe it represents a valuable and readily reproducible collection of established and emerging techniques. Such a collection is preceded by a general introductory chapter (Chap. 1) that recalls the history of immunocytochemistry, its basic principles, and its application to the study of neuronal complexity. The methods presented include immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Although this book is primarily devoted to approaches for analysis of the mammalian brain, a few nonmammalian species are also taken into consideration to demonstrate the importance of alternative animal models in a more comprehensive analysis of central and peripheral neurons. 

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