Antigen description

Antigen name Antigen description cycles screened clones clones scFvsb... 

Antigen name Antigen description Selection methods... 

Wirsching, P, et al., 1995. Reactive immunization. Science 270 1775-1783. Description of reactive immunization, in which a highly reactive compound is used as antigen. Antibodies raised against such an antigen show catalytic activity for tlie chemical reaction that the antigen undergoes. 

The following is a generic description of the immobilized antigen ELISA (Figure 2), commonly termed indirect competitive immunoassay, on a microtiter plate. 

The validity of this division within memory populations has been supported by descriptions of two populations of CD4+ memory T cells in mice, one found in lymph nodes and producing high levels of IL-2 in response to antigen... 

Van Regenmortel, M. H. V. (1996), Mapping epitope structure and activity from one-dimensional prediction to four-dimensional description of antigenic specificity , Methods Companion Meth. 

The aim of this chapter is to discuss fluorescence concepts that are used in selected immunoassay applications. The primary focus is on fluorescence topics of recent interest that provide insight into the characteristic properties of antibodies and antigens in immunoassays, or that describe enhancements in immunoassay technologies. The basic reagents and instrumentation required for immunoassay purposes are discussed first, followed by a brief description of immunoassay formats. The principles that are utilized in various fluorescence immunoassay technologies are outlined with specific examples and their significance. Since it is beyond the scope of this chapter to review all of the applications of fluorescence immunoassays, apologies are extended to authors that this chapter fails to cite. A number of comprehensive treatments of fluorescence immunoassay (FIA) applications and related topics are available. 18 ... 

Compare and contrast the different types of antibody immunoglobulins. Provide a detailed description of the structure of the IgG antibody with particular reference to how it binds to antigens. 

In any battle, when the defence is outnumbered by the enemy, more troops are brought into the battle from the reserve. However, in the immune system, there are initially no reserve troops. When an antigen binds to its complementary antibody-receptor on B-cells, these are strongly stimulated to proliferate (clonal expansion). In addition, not only does the number of daughter cells increase but each quickly develops into what is known as an effector (or plasma) cell, in which the protein synthetic machinery increases through the development of the rough endoplasmic reticulum, so that there is a large increase in the number of antibodies synthesised and secreted. A simple description of the sequence of events is as follows ... 

A. General description Muromonab-CD3 is a murine monoclonal antibody (MW 150 kDa) to the CD3 antigen of human T cells. It functions as an immunosuppressant. The antibody is a biochemically purified IgG2a immunoglobulin... 

A. General description Rituximab is a genetically engineered murine/human chimeric monoclonal antibody directed against the CD20 antigen found on the... 

Figure 7.78 Postulated mechanism of halothane immune-mediated hepatotoxicity. This figure is only a partial explanation, involving Tc cells (cytotoxic lymphocytes). See text for complete description. CYP2E1 in liver cell activates the halothane to a reactive acyl chloride shown), which reacts with proteins (e.g., enzymes in the SER). These are transported to cell surface and presented to immune system by APC. Abbreviations APC, antigen-presenting cell SER, smooth endoplasmic reticulum MHCII, major histocompatability complex.

The ELISA procedure recently has been used for the analysis of parathion (31). Since this procedure has considerable potential a more detailed description of the analysis of parathion is in order. The conjugation procedure using amino parathion (AP) was described earlier (Fig. 3, Rn 9), and this conjugate was then administered to rabbits for development of a population of specific antibodies (Abi) against BSA or AP. Abi demonstrated immunological activity only for the hapten when AP was conjugated to rabbit serum albumin (RSA). This antigen (RSA-AP) was rendered insoluble via attachment to the polystyrene surface of microtiter plates under basic conditions (Fig. 6.1). 

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