Antichymotrypsin

CRP , complement , PLA2 , serum amyloid A , fibrinogen , c -acid glycoprotein , IL-1Ra , ceruloplasmin , ai-antichymotrypsin , LBP albumin , haptoglobin , IGF-1 , transferrin , u2-HS glycoprotein ... 

Chen, Z., Prestigiacomo, A., Stamey, T. Purification and characterization of prostate-specific antigen (PSA) complexed to alpha-1 antichymotrypsin Potential reference material for international standardization of PSA immunoassays. Clin. Chem. 41, 1273-1282 (1995). 

Tysoe, C., Galinsky, D., Robinson, D., et al. (1997) Analysis of alpha-1 antichymotrypsin, presenilin-1, angiotensin-converting enzyme, and methylenetetrahydrofolate reductase loci as candidates for dementia. Am. J. Med. Genet., 74, 207-212. 

Histiocytic tumors Lysozyme, a-1-antitrypsin, a-l-antichymotrypsin, MAC-387, CD68... 

Malignant fibrous histiocytoma a-1 -antitrypsin, a-1 -antichymotrypsin, CD68, FXllla... 

ANTIBONDING ORBITAL MOLECULAR ORBITAL BONDING ORBITAL ORBITAL Antichymotrypsin,... 

The majority of hK3 (PSA) in the prostate and seminal plasma is in its free form, with less than 5% complexed with protein C inhibitor [111]. In serum, the majority of hK3 binds to protease inhibitors, and only 15%-25% is in the free form. The hK3-ai-antichymotrypsin complex constitutes 70%-85% of total serum hK3, with the hK3 2-macroglobulin and hK3 o,-antitrypsin complexes representing 15% and 3%, respectively [112-114],... 

Stenman UH, Leinonen J, Alfthan H, Rannikko S, Tuhkanen K, Alfthan O. A complex between prostate-specific antigen and alpha 1-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer Assay of the complex improves clinical sensitivity for cancer. Cancer Res 1991 51 222-226. 

Lilja H, Christensson A, Dahlen U, et al. Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin. Clin Chem 1991 37 1618-1625. 

Christensson A, Bjork T, Nilsson O, et al. Serum prostate specific antigen complexed to alpha 1-antichymotrypsin as an indicator of prostate cancer. J Urol 1993 150 100-105. 

Hutchinson S, Luo LY, Yousef GM, Soosaipillai A, Diamandis EP. Purification of human kallikrein 6 from biological fluids and identification of its complex with alpha(l)-antichymotrypsin. Clin Chem 2003 49 746-751. 

Huang Y, Herman MM, Liu J, et al. 1997. Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer s disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates. Brain Res 771 213-220. 

Antithrombin is a member of the SERPIN superfamily of proteins, which includes the inhibitors a2 an1 Pbsniin, ar antichymotrypsin, and a -proteinase inhibitor (79). Antithrombin is considered to be the primary inhibitor of coagulation (80) and targets most coagulation proteases as well as the enzymes trypsin, plasmin, and kallikrein (81). Inhibition takes place when a stoichiometric complex between the active site serine of the protease and the ARG393-SER394 bond of antithrombin forms (82,83), The tertiary structure of antithrombin resembles a,-antitrypsin in that it is folded into N-terminal domain helices and (3-sheets. This tertiary structure is maintained by the formation of three disulfide bonds (71). Four glycosylation sites exist on human... 

In cells tested, the Bowman-Birk inhibitor is more potent than the Kunitz inhibitor in decreasing cell growth. Thus, it appears that the antichymotrypsin activity is modulating growth of these cells. 

The histopathological characteristics of the brain in Alzheimer s diseases (AD) are the presence of intraneuronal neurofibrillary tangles (NFTs), extraneuronal amyloid-rich senile plaques, and a massive loss of neurons of the telencephalon. Although amyloid proteins are unique to senile plaques, several components are common to both senile plaques and NFTs hyperphosphory-lated tau proteins, ubiquitin, a 1-antichymotrypsin, apolipoprotein E, heparan sulfate proteoglycans, and Al and Fe [29]. Recent reports on Al and Fe transport mechanisms and the relation between them in AD are reviewed below. 

B14. Beatty, K., Bieth, J., and Travis, J., Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin. J. Biol. Chem. 255, 3931-3934 (1980). 

If the relationship between the concentration of the protein in the dilutions and the height of the precipitate does not give a straight line, it is often possible to obtain a straight line by further dilution of the samples and the use of less antiserum. But the limits of sensitivity for the rocket technique are determined by the formation of visible precipitin lines and this depends on the protein under study (for example, a weaker precipitin line is obtained for human serum aj-antichymotrypsin than for human serum approteinase inhibitor in the same concentration range [5]). 

Carry out the first dimension electrophoresis with 10 V/cm in the gel (controlled with the voltmeter). It ukes 80 min for the colored bovine albumin to cross the plate. This electrophoresis time was convenient in our studies of the microheterogeneity of human serum Oj-antichymotrypsin see Note 5). 

The time quoted for the first dimension electrophoresis is suitable for proteins that migrate relatively fast, like aj-antichymotrypsin. For glycoproteins with a low electrophoresis mobility, this time should be lengthened and the appropriate time for the first dimension run should be determined in initial trial experiments. 

Bowen,M.,Raynes,J G,and Cooper,E H. (1986) Changesin therelativeamountof individual microheterogeneous forms of serum Oi-antichymotrypsin in disease, in Lectins (Bog-Hansen, T C, ed.), Walter de Gruyter, Berlin-New York, pp. 403-411. 

Hachulla, E, Laine, A., and Hayem, A. (1988) ai-Antichymotrypsin microheterogeneity m crossed immunoaffinoelectrophoresis with free concanavalin A A useful diagnostic tool in inflammatory syndrome. Gbn. CSiem. 34,911-915. 

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