Antibody scFv antibodies

Antibody, scFv, against phytochrome Suspension Nicotiana tabacum (tobacco) A. tumefaciens transformation of leaf explant CaMV 35S Tobacco pathogenesis related protein (PRla) 0.5 mg L-1 (e) 5.0% of total medium protein (e) 73... 

Antibody, scFv, against carcinoembryonic antigen Callus Oryza sativa (rice) Microparticle bombardment of callus Maize ubiquitin-1 Murine heavy-and light-chain IgG 0.45 jig g"1 fresh weight without KDEL (i) 3.8 jug g-1 fresh weight with KDEL (i) 31... 

USE OF RECOMBINANT scFv ANTIBODIES TO DISRUPT FLAVONOID METABOLISM IN TRANSGENIC PLANTS... 

Single-chain antibody (scFv) Comprises a VL linked to a VH chain via a polypeptide linker. It is thus a univalent functioning antibody containing both of the variable regions of the parent antibody. 

The total cDNA construct of NPN43C9 was expressed efficiently in E. coli cells and protein purified, and its catalytic properties were assessed in both the monoclonal antibody and the single-chain antibody (scFv) 7A4-1/212 for the hydrolysis of p-nitroanilide [50] and the related p-chlorophenyl ester [51a] (Fig. 19). Virtually identical kca, and Km values were obtained for both 7A4-1/212 and NPN43C9. This activity was decreased in both cases by the addition of the inhibitor w-nitroanilide [52], which gave K = 800 p.M and 400 pM for the NPN43C9 and 7A4-1/212, respectively. 

Figure 22 Penam sulfone 39, a mechanism-based inhibitor of /3-lactamase, used as a hapten to generate scFv antibodies, FT6 and FT12, with a /3-lactamase activity.

Immunotoxins Antibody or antibody fragment (scFv, Fab, F(ab )2) Bacterial or plant toxin 3B3-PE ciTac-DT BerH2-SAP OM124-PAP [114] [115] [116.117] [117.118]... 

Moghaddam, A., Borgen, T., Stacy, J., Kausmally.L., Simonsen, B., Marvik, O. J., et al. (2003) Identification of scFv antibody fragments that specifically recognise the heroin metabolite 6-monoacetyl-morphine but not morphine. /. Immunol. Meth. 280, 139-155. 

Hust, M., Maiss, E., Jacobsen, H.-J., and Reinard, T. (2002) The production of a genus specific recombinant antibody (scFv) using a recombinant Potyvirus protease. /. Virol. Meth. 106, 225-233. 

Garrett, S.D., DJ.A. Appleford, G.M. Wyatt, et al. 1997. Production of a recombinant anti-parathion antibody (scFv) stability in methanolic food extracts and comparison to an anti-parathion monoclonal antibody. J. Agric. Food Chem. 45 4183—4189. 

Daugherty, P. S., Chen, G., Iverson, B. L., and Georgiou, G. (2000b). Quantitative analysis of the effect of the mutation frequency on the affinity maturation of scFv antibodies. Proc. Natl. Acad. Sci. USA 97, 2029-2034. 

Subsequently, it was demonstrated that it is possible to select and evolve scFv antibody fragments from immune libraries using the E. coli system. Only three rounds of ribosome display were necessary to isolate a family of scFv fragments binding to a peptide variant of the GCN4 leucine zipper, which exists as a random coil in solution (Hanes et al.,... 

The eukaryotic ribosome display was subsequently used for selection of human antibody scFv fragments binding progesterone from a library prepared from transgenic mice (He etal., 1999). In this case, a proofreading polymerase was used for the PCR amplification steps included in the ribosome display protocol. It was thereby demonstrated that it is possible to use ribosome display as a method exclusively for selection by virtually maintaining the original library repertoire. 

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