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Ligation of pHEN-1 and insert antibody scFv

Determine the size of the library by plating tenfold dilutions of transformed TGI cells from 10 to 10" onto 2 x TY-AMP-GLU agar plates. Assess background vector religation by ligating and transforming triple digested pHEN-1 vector in the absence of antibody scFv insert. [Pg.55]

See other pages where Ligation of pHEN-1 and insert antibody scFv is mentioned: [Pg.51] [Pg.504] [Pg.51] [Pg.500] [Pg.51] [Pg.504] [Pg.51] [Pg.500] [Pg.51] [Pg.51] [Pg.55]

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And insertion

Antibody scFv antibodies

Ligate

Ligation

Ligator

ScFv antibodies