ScFv antibodies

USE OF RECOMBINANT scFv ANTIBODIES TO DISRUPT FLAVONOID METABOLISM IN TRANSGENIC PLANTS... 

Figure 22 Penam sulfone 39, a mechanism-based inhibitor of /3-lactamase, used as a hapten to generate scFv antibodies, FT6 and FT12, with a /3-lactamase activity.

Moghaddam, A., Borgen, T., Stacy, J., Kausmally.L., Simonsen, B., Marvik, O. J., et al. (2003) Identification of scFv antibody fragments that specifically recognise the heroin metabolite 6-monoacetyl-morphine but not morphine. /. Immunol. Meth. 280, 139-155. 

Daugherty, P. S., Chen, G., Iverson, B. L., and Georgiou, G. (2000b). Quantitative analysis of the effect of the mutation frequency on the affinity maturation of scFv antibodies. Proc. Natl. Acad. Sci. USA 97, 2029-2034. 

Subsequently, it was demonstrated that it is possible to select and evolve scFv antibody fragments from immune libraries using the E. coli system. Only three rounds of ribosome display were necessary to isolate a family of scFv fragments binding to a peptide variant of the GCN4 leucine zipper, which exists as a random coil in solution (Hanes et al.,... 

Marty, C. Odermatt, C.M. Schott, H. Neri, D. Ballmer-Hofer, K. Klemenz, R. Schwendener, R.A. Cytotoxic targeting of F9 teratocarcinoma tumors with anti-ED-B fibronectin scFv antibody modified liposomes. British Journal of Cancer 2002, 87, 106-112. 

Marty C, Schwendener RA (2004) Cytotoxic tumor targeting with scFv antibody-modified liposomes. In Ludewig B, Hoffmann MW (eds) Adoptive immunotherapy methods and protocols. Methods in Molecular Medicine, vol 109. Humana, Totowa, NJ, pp 389 01... 

Synthesis of the diabody was carried out by means of a modification of the method reported in Refs [4.17, 4.18] for synthesis of the ior-CEAI murine antibody scFv fragment. A non-covalent dimer association was obtained from two identical ior-CEAI scFv antibody fragments, in which VL and Vl domains were linked via a 5 amino acid residue. A hexahistidine tag at the C-terminus of the Vl domain aided protein purification. 

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