Proteomics antibody

Uhlen M, Bjorling E, Agaton C, et al. A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol. Cell. Proteomics 2005 4 1920-1932. 

Koga H, Shimada K, Hara Y, et al. (2004) A comprehensive approach for establishment of the platform to analyze functions of KIAA proteins generation and evaluation of anti-mKIAA antibodies Proteomics 4, 1412-1416. 

Protein measurement [66, 67] Western blots with appropriate antibodies Proteomics Identification often uncertain, needs integration with other techniques Proteomic techniques need latest analytical tools... 

Persson A, Hober S, Uhlen M. A human protein atlas based on antibody proteomics. Current Opinion in Molecular Therapeutics. 2006 8(3) 185-90. 

Kovacs A, Sperling E, Lazar J, Balogh A, Kadas J, Szekrenyes A, et al. Fractionation of the human plasma proteome for monoclonal antibody proteomics-based biomarker discovery. Electrophoresis 2011 32(15) 1916—25. 

Jonsson H, Schiedek D, Grosvik BE, Goksoyr A (2006) Protein responses in blue mussels (Mytilus edulis) exposed to organic pollutants a combined CYP-antibody/proteomic approach. Aquat Toxicol 78 S49-S56... 

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

He, M. and Khan, F. 2005. Ribosome display next generation display technologies for production of antibodies in vitro. Expert Review of Proteomics 2(3), 421-430. 

Antibody coverage of the human proteome is estimated to be about 5 to 10% of all human proteins and isoforms (Valle and Jendoubi, 2003). A major bottleneck in the use of protein expression arrays is the lack of such a comprehensive set of these capture agents (Hanash, 2003). Since an equivalent of the polymerase chain reaction (PCR) process for mass amplification of low abxmdant proteins does not exist, the remaining library of proteome capture ligands will need to be generated by other means such as recombinant protein expression systems (Cahill, 2001). 

However, recombinant antibodies may be less stable and have lower binding affinities than monoclonal antibodies (Valle and Jendoubi, 2003). Therefore, in order to fully implement the microarray format, a host of diverse capture agents could be required in addition to antibodies. These include peptides, small molecules, aptamers, ribozymes, or other molecular recognition probes yet to be discovered. However, it is also xmderstandable because of the diverse nature of proteins that additional technologies besides microarrays will be used in proteomics research (Hanash, 2003). 

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