Antibody fingerprints

To investigate this, we constructed glycan arrays to display carbohydrate antigens of defined structures and then applied these tools to detect carbohydrate-specific antibody fingerprints that were elicited by a SARS vaccine. 

The purpose of the specificity test is to differentiate a particular molecule from other molecules that have potentially similar profiles and also to demonstrate there are no interfering peaks introduced from the sample matrix. Figure 24 shows the cIEF profiles of four different monoclonal antibodies analyzed using the same cIEF method. The profiles are significantly different. Since cIEE provides high resolution, it typically provides results that are molecule specific, and is useful as a fingerprint method. In the case where two molecules have similar p7 values and profiles, an alternative identity method is required. 

A new technique based on unique individual antibody profiles offered an alternative to current DNA fingerprinting methods. The method is simple to use ami has attracted considerable attention from law enforcement. 

Kramer, A., Vakalopoulou, E Schleuning, W.-D., and Schneider-Mergener, J. (1995) A general route to fingerprint analyses of peptide-antibody interactions using a clustered amino acid peptide library comparison with a phage display library. Mol. Immunol. 32,459 165. 

The problem of the origin of antibody diversity runs headlong into the requirement for minimal function. A primitive system with only one or a few antibody molecules would be like the propeller turning at one revolution per day not sufficient to make a difference. (More to the point, it would be as if the FBI national identification database only contained two sets of fingerprints. Out of hundreds of thousands of criminals, the FBI could only hope to catch those two.) Because the likelihood is so... 

Determined by BslNI restriction fingerprinting that was carried out on part of the candidates for binders that were identified in the initial screen b Affinity was not determined for all the selected antibodies Clones isolated by the colony lift screen... 

Figure 14.8 (a) Visual image of Jurkat cells treated with the anti-Fas DX2 monoclonal antibody (mAb) under fluorescence illumination with the IR microscope. The green and red fluorescence signify early and late apoptosis, respectively (b) A comparison of the fingerprint region of untreated cells (AV7PF) (—) with those treated with the... 

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