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Fingerprint method

Valence band spectra provide information about the electronic and chemical structure of the system, since many of the valence electrons participate directly in chemical bonding. One way to evaluate experimental UPS spectra is by using a fingerprint method, i.e., a comparison with known standards. Another important approach is to utilize comparison with the results of appropriate model quantum-chemical calculations 4. The combination with quantum-chcmica) calculations allow for an assignment of the different features in the electronic structure in terms of atomic or molecular orbitals or in terms of band structure. The experimental valence band spectra in some of the examples included in this chapter arc inteqneted with the help of quantum-chemical calculations. A brief outline and some basic considerations on theoretical approaches are outlined in the next section. [Pg.388]

Maltseva O, P Oriel (1997) Monitoring of an alkaline 2,4,6-trichlorophenol-degrading enrichment culture by DNA fingerprinting methods and isolation of the responsible organism, haloalkaliphilic Nocardioides sp. strain M6. Appl Environ Microbiol 63 4145-4149. [Pg.273]

With recent developments in analytical instrumentation these criteria are being increasingly fulfilled by physicochemical spectroscopic approaches, often referred to as whole-organism fingerprinting methods.910 Such methods involve the concurrent measurement of large numbers of spectral characters that together reflect the overall cell composition. Examples of the most popular methods used in the 20th century include pyrolysis mass spectrometry (PyMS),11,12 Fourier transform-infrared spectrometry (FT-IR), and UV resonance Raman spectroscopy.16,17 The PyMS technique... [Pg.322]

The purpose of the specificity test is to differentiate a particular molecule from other molecules that have potentially similar profiles and also to demonstrate there are no interfering peaks introduced from the sample matrix. Figure 24 shows the cIEF profiles of four different monoclonal antibodies analyzed using the same cIEF method. The profiles are significantly different. Since cIEE provides high resolution, it typically provides results that are molecule specific, and is useful as a fingerprint method. In the case where two molecules have similar p7 values and profiles, an alternative identity method is required. [Pg.377]

The simplest approach to this problem is to search a database for an identical , i.e., similar within certain tolerances, spectrum. This was developed for Infrared spectra (a technique ideally suited to such a fingerprinting method). The method was enhanced to include a more sophisticated statistical approach when applied to NMR spectra.In NMR spectra, variation in peak position due to concentration and temperature effects is larger than the peak width, and a more sophisticated approach is mandatory. In either case, the method is clearly one which yields limited or even confusing information for novel compounds. [Pg.237]

A new technique based on unique individual antibody profiles offered an alternative to current DNA fingerprinting methods. The method is simple to use ami has attracted considerable attention from law enforcement. [Pg.216]

Fingerprinting methods such as the anthocyanin methods and the Kirksey method for polyphenols (Kirksey el al., 1995) offer good ways to check for the addition of other fruits in a product. As the adulterators have become more sophisticated in the approaches that they use to extend juices, there has been a need for more complex methods of analysis. This means that it is now not uncommon to have to use fingerprinting techniques and isotopic methods to detect the most sophisticated forms of adulteration. These sophisticated analytical methods can even involve detection of the isotope ratios within a class of compounds such as sugars (Hammond el al., 1998). Using the RSSL 13C-IRIS approach, which was developed with financial support from the UK Food Standards Agency, it was possible to reduce the detection limit for the addition of C4-derived sugars to juices by about a factor of two. [Pg.271]

The decisive experiment of Biebricher and Luce is shown in Fig. 4. A synthesis medium containing highly purified enzyme and substrates is incubated and maintained at a suitable temperature, for a time adequate to allow the multiplication of any templates present but too short to enable products to arise de novo. Then the solution is divided into portions. Each portion is incubated long enough to allow synthesis de novo and the products are compared by the fingerprint method. If the impurity hypothesis is correct, then multiplication of the impurity in the first phase should lead to the same product from each portion of the incubated medium. If the de novo hypothesis is correct then the products should be different, since at the beginning different enzyme molecules were working on different products. Selection, that is, preferential reproduction of one rudimentary strand, could not yet take place, since in the first, short incubation none of the products de novo was complete. [Pg.126]

Fig. 4. A solution of nucleotide triphosphate is incubated in the presence of QP replicase for just long enough to assure the manifold replication of any templates that may contaminate the enzyme. The incubation is interrupted before even one template has time to arise de novo. The solution is then divided up into portions and the incubation is continued, this time long enough to allow products to arise de novo and to multiply. The RNA formed in each portion is analyzed by the fingerprint method various different reaction products are found. Sometimes the growth curve displays the appearance of a new mutant. Although the incubation time of template-instructed synthesis is determined unambiguously (because of the superposition of many individual processes) the synthesis de novo shows a scatter of induction times. This indicates that the initiation step is a unique molecular process which is then rapidly amplified. ... Fig. 4. A solution of nucleotide triphosphate is incubated in the presence of QP replicase for just long enough to assure the manifold replication of any templates that may contaminate the enzyme. The incubation is interrupted before even one template has time to arise de novo. The solution is then divided up into portions and the incubation is continued, this time long enough to allow products to arise de novo and to multiply. The RNA formed in each portion is analyzed by the fingerprint method various different reaction products are found. Sometimes the growth curve displays the appearance of a new mutant. Although the incubation time of template-instructed synthesis is determined unambiguously (because of the superposition of many individual processes) the synthesis de novo shows a scatter of induction times. This indicates that the initiation step is a unique molecular process which is then rapidly amplified. ...
Fig. 5. In the experiment, carried out by Charles Weissmann and his co-workers, single Qp-RNA molecules (or viruses) from a wild-type distribution (a) were cloned in E. coli bacteria. After rapid multiplication the clones of individual RNA molecules (b) were analyzed and compared by the fingerprint method. Differences were noticed in one or two positions in the sequence. After a further, long period of reproduction (c) the wild-type distribution (d) was found in every clone, that is, the average sequences had become identical again. Fig. 5. In the experiment, carried out by Charles Weissmann and his co-workers, single Qp-RNA molecules (or viruses) from a wild-type distribution (a) were cloned in E. coli bacteria. After rapid multiplication the clones of individual RNA molecules (b) were analyzed and compared by the fingerprint method. Differences were noticed in one or two positions in the sequence. After a further, long period of reproduction (c) the wild-type distribution (d) was found in every clone, that is, the average sequences had become identical again.
Epe B, Hegler J (1994) Oxidative DNA damage endonulease fingerprinting. Methods Enzymol 234 122-131... [Pg.500]

Numerous examples of 3D fingerprint methods have been described in the literature, but in this review we focus only on those which can be classified as software packages or parts of them. [Pg.40]

Kelly, M.D., Mancera R.L., Expanded interaction fingerprint method for anlays-ing ligand binding modes in docking and structure-based dmg design. J. [Pg.205]


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