Antibodies delivery

Saltzman, W.M., et al. 2000. Long-term vaginal antibody delivery Delivery systems and biodistributions. Biotechnol Bioeng 67 253. 

Flessner MF, Dedrick RL. Monoclonal antibody delivery to intraperitoneal tumors in rats effects of route of administration and intraperitoneal solution osmolality. Cancer Res 1994 54(16) 4376-84. 

Saltzman, W. M., Sherwood, J. K., Adams, D. R., Castle, P., and Haller, P. (2000), Longterm vaginal antibody delivery Delivery systems and biodistribution, Biotechnol. Bioeng., 67, 253-264. 

Transient water pores in cellular membranes are involved in several relevant processes, such as maintenance of osmotic balance, drug and antibody delivery into cells, and ion transport across the membrane. Understanding ion transport across membranes is especially important, because membranes strive to maintain a cationic electrochemical gradient used for ATP synthesis. Yet, ions leak through lipid membranes, and understanding the mechanisms associated with ion leakage would allow one to control membrane properties better in related applications. 

Identification of new target molecules to assist intracellular antibody delivery... 

Identification of New Target Molecules to Assist Intracellular Antibody Delivery... 

Fig. 2. Fast-acting acid-triggered bispecific antibody delivery systems. These new acid-triggered bispecific antibody carriers are similar to those shown in Fig. 1. However, the MAb that binds effector is directed against a conformational epitope that is altered by the mildly acidic conditions found in endosomes. Such pH-sensitive effector molecules are typified by diphtheria toxin and certain cell-binding defective mutant CRM forms of that toxin. This bispecific antibody-ligand complex spontaneously dis-...

Tian WM, Zhang CL, Hou SP, Yu X, Cui FZ, Xu QY, Sheng SL, Cui H, Li HD. Hyaluronic acid hydrogel as Nogo-66 receptor antibody delivery system for the repairing of injured rat brain in vitro. J Control Release 2005 ... 

S. Doronina, B. Mendelsohn, T. Bovee, C. Caweny, S. AUey, D. Meyer, E. Oflazoglu, B. Toki, R. Sanderson, R. Zabinski, A. Wahl, P. Senter, Enhanced activity of monomethylauristatin F through monoclonal antibody delivery effects of linker technology on efficacy and toxicity, Bioconjug. Chem. 17 (2006) 114-124. 

Frenkel, D., Solomon, B., 2002. Filamentous phage as vector-mediated antibody delivery to the brain. Proc. Natl. Acad. Sd. U. S. A. 99, 5675-5679. 

The field of DNA vaccination started when eukaryotic expression vectors were injected into the muscle of laboratory animals [2]. The authors observed protein expression for more than 2 months after injection and noted that no special delivery system was required to obtain this expression. Subsequently, it was demonstrated that antibodies can be induced simply by injecting plasmid DNA into the muscle of mice [3]. Subsequent studies found that the injection of expression plasmids also leads to the induction of a cytotoxic T-cell response. After injection, the DNA enters cells of the vaccinated host and the encoded gene becomes expressed. This eventually leads to the induction of a cellular cytotoxic T-cell, T-helper, and/or humoral (antibody) immune response. 

The "stealth" concept may offer two other opportunities for liposome application (1) Conventional immunoliposomes (see Sec. VI.C) have been shown to be removed rapidly firom the circulation by the MPS (Peeters et al., 1987). The combination of the stealth approach for longer circulation with the attachment of antibodies or antibody fragments may provide a means of delivery of drugs to their sites of action with a high degree of specificity. This could be useful for treating leukemia, graft-vs.-host diseases, and HIV disease. 

In macromolecular dmg delivery systems, dmgs are attached to polymeric compounds, such as synthetic polymers [60], dendrimers [61], and antibodies [62], in order to enhance the delivery of the active substance to the diseased tissue and to reduce the toxicity to healthy tissue. The use of macromolecular delivery systems provides several advantages extension of the half-life of the dmg, the ability to introduce targeting moieties into the carrier, the possibility of triggered dmg release, and the aforementioned reduced cytotoxicity. 

Tomlinson E. Davis S.S. (eds) (1986) Site Specific Drug Delivery. Chichester John Wiley. (This deals in part with monoclonal antibodies.)... 

Humoral antibodies of the IgG elass are able to eross the placenta flxm mother to fetus. These antibodies will provide passive proteetion of the new-born against those diseases which involve humoral immunity and to which the mother is immune. In this fashion, new-born infants in the UK have passive proteetion against tetanus but not against tuberculosis which requires cell-mediated immunity. Seeretory antibodies are also passed to the new-born together with the first deliveries of breast milk (colostrum). Such antibodies provide some passive protection against infections of the gastrointestinal tract. 

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