Anti-adenoviral antibodies

Isotype-specific anti-adenoviral antibodies in patients dosed with adenoviral-based gene therapy vectors were detected using a Biacore 3000 by Abad et al. [39]. In this assay, whole intact virus was immobihzed on the sensor surface (dextran matrix) using amine coupHng chemistry. The binding of antibodies from patient blood sera (1 10 diluted) was measured by SPR sensor and ELISA. The results obtained by the SPR biosensor were consistent with those obtained using ELISA. 

Fig. 22.5. Attaching adenoviral particles to embolization coils. Platinum and hio-degradahle coils were coated with collagen to provide a surface for subsequent attachment of an anti-adenovirus antibody. The derivatized coils were then incubated with the adenoviral vector which contained the gene for green fluorescent protein...

Other studies have used an adenoviral delivery system to invoke a protective immune response in mice (Tan et al., 2003). Immimization with this form of vaccine demonstrated a rapid anti-PA antibody response at a higher level than that of the cinrent vaccine. Further, this method of delivery offered a longer protection time in comparison with that of the rPA vaccine. However, current public fears of using a virus as an immunization vector, coupled with the lack of similar findings with other model systems, make it imperative to further research this potential area of vaccine technology. 

Dose-related gene transfer was detected in all patients at both dose levels via immunohistochemistry using an anti-HSVfA monoclonal antibody. As in the initial Phase 1 trial, significant humoral responses to the recombinant adenoviral vector were seen in all five patients, with the development of high serum titers of total and neutralizing antiadenoviral antibodies within 15-20 days of vector instillation (41). 

---