Androgen metabolites

We studied by GC-MS the urinary excretion of a single patient with a documented mutation [49] and have found that the 5/3-reduced androgen metabolite excretion relative to 5a is decreased by about 80%, and 5/ -corticosteroid metabolite excretion is essentially absent [63]. To date there has no report of an adverse endocrine phenotype related to corticosteroid, mineralocorticoid, or androgen metabolism. 

The THEiTHF + 5aTHF ratio is commonly greater than 15 in these disorders, the opposite to AME syndrome. While the ratio of saturated steroids demonstrates a high 11-oxo l 1/1-hydroxy ratio, this is not the case for steroids retaining a - -4-ene structure. The F E ratio is normal or elevated (i.e., > 1 Table 5.3.9). Steroid profile analysis also typically reveals an elevated excretion of DHEA and other androgen metabolites. Clearly the excessive ACTH production resultant from an apparent cortisol deficiency is responsible for the elevated adrenal androgen production, which in turn is responsible for female virilization and other manifestations of polycystic ovary syndrome. 

Hauser B, Mugisha L, Preis A, Deschner T (2011) LC-MS analysis of androgen metabolites in serum and urine from east African chimpanzees (Pan troglodytes schweinfurthii). Gen Comp Endocrinol 170 92-98... 

Dehydroepiandrosterone has demonstrated efficacy in the improvement of mood, interest, confidence, and anxiety levels in various populations. In a recent clinical trial, it has been reported to be useful in the treatment of negative, depressive, and anxiety symptoms of schizophrenia,and in another trial, DHEA and its metabolites produced a positive effect overall on recognition memory in postmenopausal women. In fact, estrogen metabolites of DHEA had produced a positive effect on recognition memory, while androgen metabolites produced a negative effect. This pattern reversed in perceptual identification. ... 

Elevated excretion of tetrahydrocortisone, cortolones, and androgen metabolites. Extremely low excretion of tetrahydrocortisol, 5a-tetrahydrocortisol, and cortols... 

Srinivas and Srinivasulu (1993) found that heulandate layers developed with carbon disulfide-pyridine (1 1) were effective for the separation of steroid hormones,—dehydroepiandrosterone could easily be separated from mixtures of cholesterol and estradiol benzoate or testosterone phenylpropionate, and testosterone could be separated from mixtures of cholesterol and testosterone phenylpropionate or estradiol benzoate. Likewise, estradiol could easily be separated from mixtures of cholesterol and estradiol benzoate. Daeseleire et al. (1994) applied HPTLC and GC/MS for the detection of anabolic steroids used as growth promoters in illicit cattle fattening within the European market. Agrawal et al. (1995) developed a sensitive, reliable, and rapid silica gel TLC method for the separation, identification, and quantification of stereospecific androgen metabolites. 

Agrawal, A. K., Pampori, N. A., and Shapiro, B. H. (1995). Thin-layer chromatographic separation of regioselective and stereospecific androgen metabolites. Anal. Bio-chem. 224 455-457. 

THE TREND OF ANDROGEN METABOLITES AFTER OVULATION INDUCED BY FSH AND LH FRACTIONS OR BY HCG ALONE... 

In this paper we study the behavior of urinary androgen metabolites of patients without ovulatory cycle after treatment with a combination of FSH and LH fractions prepared from urine of postmenopausal women (Jutisz M. et aL C. R. Acad. Sc. Paris 259 1195, 1964) following the scheme we used in patients with the Stein-Leventhal syndrome. 

After the treatment we observed a marked decrease in urinary androgen metabolites (Table 4). 

AdrenalInsufGclency. Low excretion of glucocorticoid metabolites and adrenal androgen metabolites characterize the steroid profile. However, the different causes (Addison s disease, lipoid adrenal hyperplasia, and congenital adrenal hypoplasia) cannot be differentiated using urinary steroid profiling. 

Dehennin, L., and Matsumoto, A.M. (1993) Long-term administration of testosterone enanthate to normal men Alterations of the urinary profile of androgen metabolites potentially useful for detection of testosterone misuse in sport. Journal of Steroid Biochemistry and Molecular Biology, 44,179-189. 

---