Testosterone phenylpropionate

TESTOSTERONE PROPIONATE 20-MG -TESTOSTERONE PHENYLPROPIONATE 40-MG -TESTOSTERONE ISCAPRIOATE 40-MG -ESTRADIOL PHENYLPROPIONATE 4MG -ESTRADIOL BENZOATE 1-MG... 

GC unit. Peak identification is as follows 1, benzylalcohol 2, testosterone propionate 3, testosterone isocaproate 4, testosterone phenylpropionate 5, testosterone decanoate 6, oil matrix (b) GC analysis of the transfer (4 pd) from the micro SEC system (c) Direct GC analysis of a standard solution of the steroid esters. Reprinted from Proceedings of the 10th Symposium on Capillary Chromatography, M. Ghys et al, On-line micro size-exclusion chromatography-capillary gas chromatography, 1989, with permission from Wiley-VCH. 

Steroid hormones, especially those containing the A S-keto-chromophore group, are frequently marketed in the form of oily injections. Direct determination of the active ingredient content using the CD method is conveniently done by measuring the ellipticity of an aliquot of the injection after the very simple process of dilution with an appropriate solvent [22]. Problems that might arise in these analyses and the recommended method of examination are discussed in the case of an oily injection that contains testosterone phenylpropionate. 

In an earlier study [24], CD spectra for A -3-ketosteroids in different solvents were reported. Of the solvents studied, cyclohexane appeared to be the most suitable choice. However it was discovered that benzyl alcohol, present in the injection as a preservative at a concentration of 10% v/v, distorts the CD spectrum of testosterone phenylpropionate when cyclohexane is used as the solvent. Variations in the CD spectra of testosterone phenylpropionate in the presence of increasing concentrations on benzyl alcohol are shown in Fig. 3. 

Figure 3. CD curves for testosterone phenylpropionate injections with various concentrations of benzyl alcohol a 1.0 ml injection was diluted to 25.0 ml with cyclohexane, (a) 50pl, (b) 200 pi, (c) 600pi benzyl alcohol per 1 ml oily solution. The descending ordinate represents increasing negative ellipticity and the scale divisions are 0.01°. (From Ref. 22).

Figure 4. Dependence of the ellipticity ratio (R) of testosterone phenylpropionate on the benzyl alcohol content of injection after dilution with (a) cyclohexane and (b) dioxane. (From Ref. 22).

Components) of sunflower oil, the carrier solvent in oily injection formulations, were found to produce a negative Cotton effect below 334 nm which will interfere with that for testosterone phenylpropionate at its wavelength of maximum negative ellipticity, 330 nm. Consequently, the wavelength selected for the direct measurement of ellipticity of the steroids was 342.8 nm, the long wavelength of maximum negative ellipticity of the steroids, where the sunflower oil exhibits no CD. 

CD curves for testosterone phenylpropionate in four different solvents are shown in Figure 6. Curves for the other compounds, testosterone, methyltestosterone, different testosterone esters, nandrolone phenylpropionate, norethisterone, and d-norgestrel, are essentially coincident with those in Fig. 6. The figure also demonstrates well how an increase in solvent polarity leads to a... 

This solvent effect is the basis for the development of a difference CD spectroscopic method for the determination of A4-3-ketosteroids present in pharmaceutical formulations. Ellipticities for solutions of equal concentrations of these compounds in both cyclohexane and methanol are measured at 353.1 nm. A spectrum which is the difference between the CD curves obtained in this way for testosterone phenylpropionate is shown in Fig. 7. 

Nortestosterone phenylpropionate was found [246] to have a myotrophic effect four times stronger and an androgenic effect only half that of testosterone phenylpropionate. Nitrogen and calcium retention values were also favorable [238]. 

Sustanon (Testosterone Propionate 30 mg, Testosterone Phenylpropionate 60 mg, Testosterone Isocaproate 60mg, and Testosterone Decanoate 100 mg) 250mg/cc... 

Peaks (1) testosterone propionate, (2) testosterone isocap-roate, (3) testosterone phenylpropionate. (4) testosterone de canoate... 

Figure 60. Capillary SFC analysis of steroid esters Column 20 m LxO. I mm i.d. 0.1 pm df MeSi temperatnre 130 °C pressure 200 bar CO FID Peaks (1) testosterone propionate. (2) testosterone isocap-roate, (3) testosterone decanoate. (4) testosterone phenylpropionate...

Srinivas and Srinivasulu (1993) found that heulandate layers developed with carbon disulfide-pyridine (1 1) were effective for the separation of steroid hormones,—dehydroepiandrosterone could easily be separated from mixtures of cholesterol and estradiol benzoate or testosterone phenylpropionate, and testosterone could be separated from mixtures of cholesterol and testosterone phenylpropionate or estradiol benzoate. Likewise, estradiol could easily be separated from mixtures of cholesterol and estradiol benzoate. Daeseleire et al. (1994) applied HPTLC and GC/MS for the detection of anabolic steroids used as growth promoters in illicit cattle fattening within the European market. Agrawal et al. (1995) developed a sensitive, reliable, and rapid silica gel TLC method for the separation, identification, and quantification of stereospecific androgen metabolites. 

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