Analyze transcriptional

Analyze transcription and translations of genetic codes to proteins (see Appendix 2, Section A2.3). 

Figure 5. Role of DNA replication in the development of a transcriptionally repressive state. BrUTP incorporation of membrane-permeabilized embryos was conducted on two-cell embryos in G2 (2C) or S-phase-arrested, one-cell embryos that were analyzed at a time that corresponded to G2 of the two-cell stage. Both the male (aph M) and female (aph F) were analyzed. Transcription was also analyzed at a time that corresponded to C2 of the two-cell stage for S-phase-arrested, two-cell embryos (aphidicolin added after the first round of replication aph 2C) or in two-cell embryos treated with trapoxin (trap). The data are expressed relative to the amount of BrUTP incorporated by a two-cell blastomere nucleus in C2. (Data taken from Aoki et al., 1997).

Gordon, D. B., Nekludova, L., McCallum, S., and Fraenkel, E. (2005) TAMO a flexible, object-oriented framework for analyzing transcriptional regulation using DNA-sequence motifs. Bioinformatics 21, 3164-3165. 

We hope that the instructions and protocols provided here will encourage other investigators to begin analyzing transcription in Drosophila on a genomic scale. In the coming... 

Cytokine profiling has also been measured as a function of changes in cytokine mRNA expression using either reverse transcription polymerase chain reaction (RT-PCR) [87, 91-93] or ribonuclease protection assay (RPA) [94-97], Measurement of cytokine transcripts by RT-PCR revealed that prolonged exposure to TMA induced increased levels of IL-4 mRNA expression compared with treatment with DNCB [87,92-93]. However, expression of the type 1 cytokine IFN-y by DNCB-activated LNC was variable and failed to discriminate between contact and respiratory allergens [87,91,93). A similar profile was observed for freshly isolated tissue analyzed by RPA. This somewhat less... 

Numerous studies have analyzed the regulatory effects of RNI on transcription factors and transcriptional regulation. Although outside the main focus of this article, to summarize these primary observations we provide a table showing several transcription factors and their regulation under the impact of RNI (Table 13.1). For extensive coverage of the primary literature we refer to a number of review articles [11-14],... 

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