Analysis of nucleic acids

Fluorescence Methods for the Analysis of Nucleic Acids in Recombinant Biological Products... 

Ion exchange chromatography has also been extensively applied to the analysis of nucleic acid components (3,14,35),... 

There exist numerous reports on the interaction of noble metal nanoparticles, especially those of gold, with DNA. The reason for this intense work in most cases is the use of gold nanoparticles for the analysis of nucleic acids, or proteins. In any case, the interactions between the two... 

Comparative sequence analysis based on MALDI-TOF-MS analysis of nucleic acids cleaved at specific bases and reference sequences used to construct in silico cleavage patterns enable cross-correlation of theoretical and experimental mass signal patterns. Observed signal pattern differences are indicators of sequence variations and... 

For molecular sizes that are amenable by NMR techniques, nucleic acids usually lack a tertiary fold. This fact, together with the characteristic low proton density, complicates NMR structural analysis of nucleic acids. As a result, local geometries and overall shapes of nucleic acids, whose structures have been determined by NMR, usually are poorly defined. Dipolar couplings provide the necessary long-range information to improve the quality of nucleic acid structures substantially [72]. Some examples can be found already in the literature where the successful application of dipolar couplings into structure calculation and structure refinement of DNA and RNA are reported [73-77]. 

Ding C, Cantor CR. 2004. Quantitative analysis of nucleic acids— the last few years of progress. J Biochem Mol Biol 37(1) 1. 

Willems, A. V., Deforce, D. L., Van Peteghem, C. H., and Van Bocxlaer, J. E. (2005). Analysis of nucleic acid constituents by on-line capillary electrophoresis-mass spectrometry. Electrophoresis 26, 1221-1253. 

Klostermeier, D. and D. P. Millar. Time-resolved fluorescence resonance energy transfer A versatile tool for the analysis of nucleic acids. Biopolymers 61, 159-179 (2002). 

The observation of currents attributable to the faradaic electrochemistry of nucleic acids was pioneered by Palecek and coworkers who studied DNA adsorbed on mercury or carbon electrodes [13]. The signals detected by Palecek were attributable to oxidation of the purines, which produced signals indicative of irreversible processes involving adsorbed bases. These reactions were used as a basis for electrochemical analysis of DNA. Kuhr and coworkers later showed that similar strategies could be developed for analysis of nucleic acids via oxidation of sugars at copper electrodes [14-16]. 

Rosenbaum V, Reissner D (1987) Temperature gradient electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophys Chem... 

The amino acid sequence of a protein is determined by identifying amino acid residues as they are sequentially cleaved from the intact protein (see Experiment 2). Sequence analysis of nucleic acids is based on the generation... 

E. Palecek, M. Fojta, F. Jelen and V. Vetterl, Electrochemical analysis of nucleic acids. Bioelectrochemistry. In A.J. Bard and M. Stratmann (Eds.), The Encyclopedia of Electrochemistry, Vol. 9, Wiley-VCH, Weinheim, Germany, 2002, pp. 365-429 Chap. 12, and references therein. 

Because conformational changes in RNA or short DNAs typically cause small changes in electrophoretic mobility, analysis of nucleic acid folding requires careful optimization of electrophoresis conditions. By contrast, protein—nucleic acid interactions are typically easier to analyze by native PAGE because the molecular weight and positive charge of the protein produces a relatively large shift in gel mobility. 

Komenda, J. Symp. on Electrochemical Analysis of Nucleic Acids, Lisewsky Dour, Czechoslovakia 1975, Abstracts p. 9... 

For many years, mass spectrometry has had an important role in the analysis of nucleic acids. However, older work was limited to the analysis of nucleotides because the El and Cl methods did not allow oligonucleotide analysis. With the advent of FAB and PD, small oligonucleotides comprising up to about 10 bases have been analysed. 

Heller C (ed.) (1997) Analysis of nucleic acids by capillary electrophoresis. Vieweg, Braunschweig... 

Lecoq AF, Leuratti C, Marafante E, Di Biase S (1991) Analysis of nucleic acid derivatives by micellar electrokinetic capillary chromatography. J High Resolut Chromatogr 14 667-672. 

Duveneck GL, Abel AP, Bopp MA (2002) Planar waveguides for ultra-high sensitivity of the analysis of nucleic acids. Anal Chim Acta 469 49-61... 

Horvath, C., High-Performance Ion-Exchange Chromatography with Narrow-Bore Columns Rapid Analysis of Nucleic Acid Constituents at... 

Analysis of Nucleic Acid Constituents at the Subnanomede Level (Horvath). 21 79... 

In this article we will discuss the use of reversed-phase chromatography in the analysis of nucleic acid fragments. For in-depth reviews of chromatographic theory and general applications, the reader is referred to several reviews which are available (B24, B31, G5, K8, M16, S17, S23). 

Friedman RA, Honig B. A free energy analysis of nucleic acid base stacking in aqueous solution. Biophys. J. 1995 69 1528-1535. 

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