Analysis of mitochondria

Mattiasson G, Friberg H, Hansson M, Elmer E, Wieloch T. 2003. Flow cytometric analysis of mitochondria from CAl and CA3 regions of rat hippocampus reveals differences in permeability transition pore activation. J Neurochem 87 532-544. 

One complicating factor in the analysis of mitochondria from tissue cross sections is that mitochondria are found in two locations in muscle tissue (i) subsarcolemmal mitochondria are found directly beneath the sarcolemmal membrane and are easily extracted and (ii) interfibrillar mitochondria are located between the myofibrils and require exposure to a protease to be efficiently released. To release these latter mitochondria, a short treatment with trypsin was tested by depositing a microdroplet of trypsin on the fiber before sampling. Table 20.2 compares the analysis of mitochondria from muscle fiber that had undergone trypsin treatment with the analysis of mitochondria from... 

Whiting, C. and Arriaga, E. A., CE-LIF analysis of mitochondria using uncoated and dynamically coated capillaries. Electrophoresis, 27, 4523-4531, 2006. 

Histiometric and Enzymic Analysis of Mitochondria of Locust Flight Muscle ... 

Benel, L. Ronot, X. Mounolou, J. C. Gaudemer, F. Adolphe, M. Compared flow cytometric analysis of mitochondria using 10-A-nonyl acridine orange and... 

Analysis of Mitochondria Transmembrane Potentiai, and Generation of Reactive Oxygen Species (ROS)... 

Zhao, W Waisum, O. Fung, Y Cheung, M. P. Analysis of mitochondria by capillary... 

These predictive methods are very useful in many contexts for example, in the design of novel polypeptides for the identification of possible antigenic epitopes, in the analysis of common motifs in sequences that direct proteins into specific organelles (for instance, mitochondria), and to provide starting models for tertiary structure predictions. 

Hafher, R.P., Brown, G.C.. Brand, M.D. (1990). Analysis of the control of respiration rate, phosphorylation rate, proton leak rate and proton motive force in isolated mitochondria using the top-down approach of metabolic control theory. Eur. J. Biochem. 188,313-319. 

Miyoshi, H. and Fujita, T. (1988). Quantitative analysis of the uncoupling activity of substituted phenols with mitochondria from flight muscles of house flies, Biochim. Biophys. Acta, 935, 312-321. 

Ratinaud MH, Leprat P, Julien R. 1988. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry 9 206-212. 

BT 20 cells incubated in serum free medium for 10 hours with the vector/ DNA complexes (DQAplexes, C-DQAplexes). For control, cells were exposed to naked DNA and empty vesicles. The cells were then stained with Mitotracker Red CMXRos (Molecular Probes) for five minutes to enable the visualization of mitochondria followed by confocal fluorescence microscopic analysis on a Zeiss Meta 510 Laser Scanning Microscope. 

Ozone has been shown to initiate many physiological and biochemical changes in sensitive plant species. Decreases in photosynthesis and increases and decreases in respiration have occurred in response to ozonation. The bioenergetic status of mitochondria and chloroplasts is disturbed by ozone. Decreases in oxidative- and photo- phosphorylation have been reported as have increases in adenosine triphosphate and total adenylate content of plant tissue. The variable physiological responses appear to be related to the stage of symptom development at the time of analysis and to the mode of ozone exposure, viz. in vivo and in vitro. 

Cloning and characterization of the gene for hypertension and brachydactyly on the short arm of chromosome 12 Comparative physical and transcriptional mapping of human chromosome 20ql3 segment as candidate for imprinting MITOP - the mitochondria project Molecular analysis of the vertebrate genome... 

Norepinephrine and epinephrine can be metabolized by several enzymes, as shown in Figure 6-6. Because of the high activity of monoamine oxidase in the mitochondria of the nerve terminal, there is significant turnover of norepinephrine even in the resting terminal. Since the metabolic products are excreted in the urine, an estimate of catecholamine turnover can be obtained from laboratory analysis of total metabolites (sometimes... 

Chytrid hydrogenosomes took rather different from the pictures of mitochondria in textbooks (Fig. 4). However, serial sectioning followed by electron microscopical analysis revealed a structure resembling the ultrastructure... 

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