Amplification technology

Nygaard V, Hovig E. Options available for profiling small samples a review of sample amplification technology when combined with microarray profiling. Nucleic Acids Res 2006 34 996-1014. 

Hartley DP, Klaassen CD. Detection of chemical-induced differential expression of rat hepatic cytochrome P450 mRNA transcripts using branched DNA signal amplification technology. Drug Metab Dispos 2000 28 608-616. 

In contrast to nucleic acids where polymerase chain reaction (PCR) allows the investigation of single cells, no amplification technology is available at the protein level. As such, proteomic studies require relatively large sample volume. Because of this limitation, it is not a surprising that most proteomic studies have been performed on whole tissue samples. This approach in cancer research is, however, unfortunate because cellular heterogeneity within a biopsy or tissue sample will likely impair the quality and reproducibility of information generated. 

New Array and Signal Amplification Technology with SPRi.146... 

Saldanha J, Lelie N, Heath A. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA. WHO Collaborative Study Group. Vox Sang 1999 76 149-58. 

DNA testing is relatively new in diagnostics, and amplification technology is as yet untried in a licensed format. Proficiency panels have been made and used in the reference laboratories for the research formats, and researchers have been constructing and sharing their panels for over 2 years. However, these panels, as... 

Nucleic Acid Amplification Technologies (NAATs) such as PCR, are generally followed (often in real-time) by some means to detect the accumulation of nucleic acid amplicon. However, as pyrophosphate is a by-product of nucleic acid biosynthesis, the generation of pyrophosphate can also be used to follow NAATs. 

Vaneechoutte, M., Van Eldere, J. 1997. The possibilities and limitations of nucleic acid amplification technology in diagnostic microbiology. J. Med. Microbiol. 46 188-194. 

The ability of cloned muscarinic receptors to mediate responses related to proliferation of living mammalian cells was evaluated. In this assay, ligand/receptor interactions allow the selection and amplification of NIH-3T3 cells that also express P-galactosidase (receptor selection and amplification technology, R-SAT Receptor Technologies Braeuner-Osborne, 1995 Braeuner-Osborne and Brann, 1996). 

One of the barriers to the spread of rapid amplification technology for POC clinical use is the cost of the instrumentation, which limits the use of the technology. To make biosensor technology more accessible, recently a manuscript was published (38) describing an inexpensive array biosensor. Here we describe a simple and inexpensive battery-powered thin-film... 

Rapid detection with fewer false alarms Mass production Moleculeu amplification technologies Nanocatalytic technologies rmd regenerative materieils... 

Examples of Sensors Prepared with Amplification Technology... 

The variety of fiber opUc sensors prepared with amplification technology demonstrate the generality of the method. 

---