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Rolling circle amplification technology

A novel signal amplification method based on the rolling circle amplification (RCA) technology has also been reported. RCA involves many rounds of isothermal enz5miatic... [Pg.8]

Mullenix MC, Dondero RS, Datta HD, Egholm M, Kingsmore SF, Perlee LT. Rolling circle amplification in multiplex immunoassays. In Demidov W, Broude NE, editors. DNA Amplification—Current Technologies and Applications. Norfolk, UK Horizon Bioscience, 2004 313-331. [Pg.288]

It is apparent that signal amplification provides increased sensitivity over direct labeling. This is especially true for fluorescent-based assays. One of the most sensitive signal detection technologies is the immunoRCA (Schweitzer et al., 2000). Rolling circle amplificahon (RCA) is combined with antibody detection. RCA involves the amplification of circularized oligonuceotide probes under isothermal conditions by DNA polymerase (Lizardi et al., 1998). With immunoRCA, the 5 primer is attached to the reporter antibody. Initiation of the amplification starts when circular DNA template binds to the attached primer. [Pg.212]

See other pages where Rolling circle amplification technology is mentioned: [Pg.343]    [Pg.320]    [Pg.343]    [Pg.320]    [Pg.234]    [Pg.253]    [Pg.186]    [Pg.1900]   
See also in sourсe #XX -- [ Pg.8 ]



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