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Preparation with amplification technology

Examples of Sensors Prepared with Amplification Technology... [Pg.260]

The variety of fiber opUc sensors prepared with amplification technology demonstrate the generality of the method. [Pg.260]

In the sample preparation step, the nucleic acid content from the sample is extracted, concentrated, and purified to meet the quality required by the downstream amplification process. Sample preparation of nucleic acid targeting technologies involves several processes. In most cases, lysis is an initially required process to break open intact structures of targeted species (e.g., cell wall, membrane, and capsules) and to release nucleic acids. Additional purification or extraction of the released nucleic acids from the lysate may be conducted to minimize inhibitory effects on downstream amplification. Following the lysis step, the chemicals introduced (e.g., membrane-solubilizing ionic detergents or proteinases), cell debris, and other interferents that come with samples are removed. For diluted specimens (i.e., urine), nucleic acid concentration may be required in order to reduce the limit of detection of amplification and detection assays. [Pg.147]


See other pages where Preparation with amplification technology is mentioned: [Pg.261]    [Pg.261]    [Pg.257]    [Pg.68]    [Pg.234]    [Pg.2116]    [Pg.97]    [Pg.256]    [Pg.216]    [Pg.120]    [Pg.17]    [Pg.231]    [Pg.28]    [Pg.439]    [Pg.384]    [Pg.349]    [Pg.357]    [Pg.360]    [Pg.14]    [Pg.18]    [Pg.109]    [Pg.455]    [Pg.267]    [Pg.65]    [Pg.86]    [Pg.253]    [Pg.371]    [Pg.537]    [Pg.167]    [Pg.623]    [Pg.64]    [Pg.107]    [Pg.59]    [Pg.180]    [Pg.89]    [Pg.329]    [Pg.155]    [Pg.1239]    [Pg.2684]    [Pg.105]    [Pg.3801]    [Pg.218]    [Pg.153]   
See also in sourсe #XX -- [ Pg.260 , Pg.262 , Pg.264 ]




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