Agilent Technologies

The author wishes to acknowledge the efforts of his various research students from whose work some of the applications presented here have been drawn. In particular, I have enjoyed the excellent partnership in the pioneering PhD research of Mr (now Dr) Russell Kinghom. In addition, the provision of a gas chromatograph by Agilent Technologies and columns from SGE International have enabled our work to progress. 

Figure 4.2 Schematic of a direct-liquid-introduction LC-MS interface. From applications literature published by Agilent Technologies UK Limited, Stockport, UK, and reproduced with permission.

Nanotechnology Measurements Division Agilent Technologies Chandler, Arizona... 

Column, gas chromatograph FlP-5, 5% phenyl-methyl capillary column, 0.25-mm i.d. X 12-m length, 0.33-qm film thickness (Agilent Technologies) or equivalent Evaporator, rotary vacuum, water-bath set at 60 and 40 °C Processors ... 

Gas chromatograph. Model 6890 (Agilent Technology) equipped with split/splitless injector and a mass spectrometer. Model 5973, or equivalent Tissumizer, Model SDT 1810 SI with S25N probe (Tekmar, Cincinnati, OFl, USA) or equivalent... 

Agilent Technologies Micro Fluidic Systems Naval Medical Research Center SRI... 

D. Heiger, High Performance Capillary Electrophoresis, Agilent Technologies Primer, Publ. no. 5968-9963E, Wilmington, DE (2000). 

R. Schuster and H. Schulenberg-Schell, A New Approach to Lower Limits of Detection and Easy Spectral Analysis, Agilent Technologies Application Primer No. 

Figure 7.15 Schematic of the GC-ICP-MS interface. Reproduced by permission of Agilent Technologies...

M. Yamanaka and O.F.X. Donard, Agilent Technologies Application Note (Apr. 2000). 

The LCQ and LCQ DECA are products of Thermo Electron Corporation (San Jose, CA) the Q-TOF is a product of Waters (Beverly, MA) the QSTAR, ProQUANT, and ProICAT are products of Applied Biosystems (Foster City, CA) and Spectrum Mill and the MSD TRAP XCT are products of Agilent Technologies (Palo Alto, CA). 

We typically use RNA purification by the Trizol method (Invitrogen, following the manufacturer s instructions), which has the advantage over column-based methods that it can purify small amounts of RNA and retain miRs. Purified RNA is dissolved in RJNAse-free water and stored at —80°. RNA quality is assessed on an Agilent Bioanalyzer (Agilent Technologies) or by gel electrophoresis. 

Procedure Phenolic acids were detected between 210 and 360 nm using a Hewlett Packard diode array detector (HP 1100 HPLC system). The separation was achieved with a Nucleosil 100-5 C18 column 5 pm 4.0x250 mm (Agilent Technologies, USA) at a flow rate of 1.0 ml/min and injection volume of 5 mL. For the elution, a discontinuous acetonitrile-water gradient was used 15% acetonitrile (5 min), 30% acetonitrile (20 min), 40% acetonitrile (25 min), 60% acetonitrile (30 min), 60% acetonitrile (35 min) and... 

FIGURE 3.3 Van Deemter plot of columns with different particle sizes. (Courtesy of Agilent Technologies, Inc.)... 

FIGURE 3.5 Calculated efficiency yields (fraction of efficiency with and without dispersion) for different column dimensions, always assuming sub-2-micron particles. Solid lines assumed 36-/.(L dispersion. Broken lines 8-/tl. dispersion. (Courtesy of Michael Woodman, Agilent Technologies, Inc.)... 

Agilent Technologies, Inc., Application Compendium, 2005, Publication 5989-2549EN, Time-of-Flight Solutions in Pharmaceutical Development The Power of Accurate Mass. 

Agilent Technologies introduced a Rapid Resolution Liquid Chromatography (RRLC ) system with pressure capabilities up to 600 bars. 

---