Agilent bioanalyzer

Agilent 2100 Bioanalyzer and Protein 200 plus lab-chip kit (Agilent, Palo Alto, CA). 

The LabChip is then placed in an Agilent 2100 Bioanalyzer and the Protein 200 Plus program is run. 

Fig. 1. Purified proteins imaged using (A) Agilent 2100 Bioanalyzer and (B) traditional sodium dodecyl sulfide-polyacrylamide gel electrophoresis. (A) Note that the lowest two bands and the uppermost band are internal standards for sizing and quantitation. (B) 3-15% polyacrylamide gel stained with GelCode Blue. In both panels, the predicted size of the protein is labeled at the top of each lane.

Gottwald, E., Muller, O., Polten, A., Semiquantitative reverse transcription-polymerase chain reaction with the Agilent 2100 Bioanalyzer. Electrophoresis 2001, 22(18), 4016 1022. 

The so-called micro-total analytical systems (/tTAS) can integrate sample handling, separation, and detection on a single chip [9]. Postcapillary reaction detectors can be incorporated as well [10]. Fluorescence detection is the most common method employed for these chip-based systems. A commercial instrument (Agilent 2100 Bioanalyzer) is available for DNA and RNA separations on disposable chips using a diode laser for LIF detection. In research laboratories, polymerase chain reaction (PCR) has been integrated into a chip that provides size separation and LIF detection [11]. 

Nachamkin I, Panaro NJ, Li M, Ung H, Yuen PK, Kricka LJ, et al. Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene. J Clin Microbiol 2001 39 754-7. 

LJ, Wilding P. Evaluation of DNA fragment sizing and quantification by tire agilent 2100 bioanalyzer. Clin Chem 2000 46 1851-3. 

Fig. 4.28. Bench-top Agilent 2100 Bioanalyzer" (Photograph courtesy of Agilent Technologies)...

The advent of microfluidic devices like the Agilent 2100 Bioanalyzer (Agilent Technologies, Sanata Clara, CA) has diverse implications for CE-based protein analysis. This commercially available microchip CE platform is a dedicated instmment that uses manufacturer-supplied reagent... 

Sylvie Veriac, Valerie Petrone, Madeleine Avon, Identification of red and white blood cells from whole blood samples usingthe Agilent 2100 bioanalyzer. 

Nitsche, R. (2011). Milk protein analysis with the Agilent 2100 bioanalyzer and the Agilent Protein 80 kit. Waldbronn, Germany Agilent Technologies. 

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