Agilent 5972 quadrupole

Confirmatory system Agilent Series 1100 liquid chromatograph Eclipse XDB Cig HPLC columu, 150 x 4.0-mm i.d., 3.5- im particle size MicroMass Quattro II triple-quadrupole mass spectrometer using an electrospray ionization (ESI) interface... 

LA-ICPMS analyses of pyrite and pyrrhotite in both spot and imaging mode (for 32 and 22 elements respectively) were completed on a New Wave 213nm solid-state laser microprobe coupled to an Agilent 4500 quadrupole ICPMS using the procedures otLarge etal. submitted). 

We use single-stage quadrupole (typically Agilent bench-top 5971 or 5973MSD) instruments for most applications. The current model offered is the Agilent 5975 (www. agilent.com). 

We use a GC Top 8000 gas chromatograph coupled with a PolarisQ ion-trap mass spectrometer and equipped with an AI3000S autosampler (Thermofinnigan www. thermo.com). The steroids are separated on a DB-1 crosslinked methyl-silicone column, 15 mx 0.25 mm i.d., film thickness 0.25 pm (J W Scientific marketed by Agilent). Helium is used as a carrier gas at a constant pressure of about 35 kPa. A 1-pl aliquot of the final derivatized extract is injected into the system operated in splitless mode (valve opened at 2 min). The GC temperature program is the same described before for the quadrupole GC-MS system. The injector and transfer lines are kept at 260°C and 280°C, respectively. The ion source temperature is 225°C. A damping gas flow of helium is applied to the ion trap. 

Figure 5.3 Instrumental outline of a quadrupole-based ICP-MS without collision/reaction cell (Agilent 7500). (Reproduced by permission of Agilent.)...

Collision/reaction cell Agilent ceVcs2 octopole cell (ORS) Elan DRC II quadrupole dynamic reaction cell XSeries11 hexapole cell ( XS-Minus modus) Varian-820-MS using collision reaction interface... 

Figure A.3A.3 LC/MS analysis of a dietary supplement consisting of extract of Trifolium pratense (red clover). Reversed-phase C18 HPLC and negative ion electrospray ionization mass spectrometry were used with a quadrupole mass spectrometer analyzer (Agilent also see Table A.3A.1). The map illustrates the abundance of information provided by this hyphenated technique with HPLC mass chromatograms in one dimension and mass spectra in another dimension.

Martens-Lobenhoffer et al. [119] used chiral HPLC-atmospheric pressure photoionization tandem mass-spectrometric method for the enantio-selective quantification of omeprazole and its main metabolites in human serum. The method features solid-phase separation, normal phase chiral HPLC separation, and atmospheric pressure photoionization tandem mass spectrometry. The internal standards serve stable isotope labeled omeprazole and 5-hydroxy omeprazole. The HPLC part consists of Agilent 1100 system comprising a binary pump, an autosampler, a thermo-stated column component, and a diode array UV-VIS detector. The enantioselective chromatographic separation took place on a ReproSil Chiral-CA 5 ym 25 cm x 2 mm column, protected by a security guard system, equipped with a 4 mm x 2-mm silica filter insert. The analytes were detected by a Thermo Scientific TSQ Discovery Max triple quadrupole mass spectrometer, equipped with an APPI ion source with a... 

Sample analysis was performed by using an Applied Biosystems (Foster City, CA) API 3000 triple quadrupole mass spectrometer equipped with a TurboIonSpray source and an Agilent 1100 capillary HPLC system (Palo Alto, CA). The capillary HPLC system included a binary capillary pump with an active micro flow rate control system, an online degasser, and a microplate autosampler. The analytical column was a 300 pm I.D.x 150 mm Zorbax C18 Stablebond capillary column (pore size 100 A and particle size 3.5 pm). The injection volume was 5 pL, and a needle ejection rate of 40 pL/min was used. The pLC flow rate was 6 pL/min. In order to minimize dead volume before the column, the autosampler was programmed to bypass the 8 pL sample loop 1.5 min after injection. The mobile phase consisted of (A) 2 mM ammonium acetate (adjusted to pH 3.2 with formic acid) in 10 90 acetonitrile-water, and (B) 2 mM ammonium acetate in 90 10 acetonitrile-water. The percentage of mobile phase B was held at 32 % for the first minute, increased to 80 % over 8 min, and then increased tol00% over the following 1 min. 

LC-MS/MS System Used and Analytical Conditions An Agilent Technologies (Palo Alto, CA, USA) 1100 series LC-system was used, connected to an API 4000 triple quadrupole mass spectrometer (Applied Biosystems MDS Sciex, Concord, ON, Canada). The mass spectrometer was equipped with an Electrospray ionization source (negative ion mode). 

A major breakthrough was the eommereial introduetion of dedieated quadrupole ion-trap instruments equipped with an API souree for ESI and APCI, i.e., the LCQ instraments from Thermo Fiimigan, the Esquire from Broker, and the LC-MSD-Trap from Agilent Technologies. 

A large number of manufacturers make numerous quadrupole mass spectrometer models. A few prominent companies among the many in the field include Agilent, Thermo Finnigan, Waters Micromass, and Applied Biosys-tems/MDS Sciex. Most QMFs are intended primarily for use with separations, such as GC or LC. Nearly any ion source imaginable is marketed with one quadrupole mass spectrometer or another, including El, ESI, APCI, IGF, and others. 

Figure 3.41 Agilent 1200 Series LCMS selection of detectors - ion trap, triple quadrupole and TOF with multimode source ( Copyright 2006, Agilent Technologies, Inc. Reproduced with permission).

Figure 6.10 Hybrid quadrupole trme-of-flight (QqTOF) mass analyzer. (Schematic diagram provided courtesy of Agilent Technologies.)...

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